Boekhoff I, Tareilus E, Strotmann J, Breer H
University of Stuttgart-Hohenheim, Department of Zoophysiology, FRG.
EMBO J. 1990 Aug;9(8):2453-8. doi: 10.1002/j.1460-2075.1990.tb07422.x.
The molecular mechanisms mediating the chemo-electrical signal transduction in olfactory receptor cells are still elusive. In this study odor induced formation of second messengers in rat olfactory cilia was monitored in a subsecond time range using a rapid kinetic device. Application of micromolar concentration of citralva induced a rapid, transient elevation of the cyclic adenosine monophosphate level, whereas the concentration of inositol trisphosphate was not affected. In contrast, pyrazine caused a rise in the concentration of inositol trisphosphate, not affecting the level of cyclic adenosine monophosphate. Analysis of the kinetic parameter for the odorant induced reaction indicated that apparently two systems are operating simultaneously. The activating effects of odorants appear to be mediated via different G-proteins. Thus, at least two different second messenger pathways appear to be involved in olfactory signal transduction.
介导嗅觉受体细胞中化学-电信号转导的分子机制仍不清楚。在本研究中，使用快速动力学装置在亚秒时间范围内监测了气味诱导大鼠嗅觉纤毛中第二信使的形成。应用微摩尔浓度的柠檬醛可诱导环磷酸腺苷水平迅速、短暂升高，而三磷酸肌醇的浓度未受影响。相反，吡嗪导致三磷酸肌醇浓度升高，而不影响环磷酸腺苷水平。对气味诱导反应的动力学参数分析表明，显然有两个系统同时运行。气味剂的激活作用似乎是通过不同的G蛋白介导的。因此，嗅觉信号转导中似乎至少涉及两条不同的第二信使途径。
