Lobasso Simona, Lopalco Patrizia, Angelini Roberto, Baronio Maristella, Fanizzi Francesco P, Babudri Francesco, Corcelli Angela
Department of Medical Biochemistry Medical Biology and Medical Physics, University Aldo Moro, Pl. G. Cesare, 70124, Bari, Italy.
Lipids. 2010 Jul;45(7):593-602. doi: 10.1007/s11745-010-3432-1. Epub 2010 May 29.
The use of the matrix 9-aminoacridine has been recently introduced in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis of both anionic and cationic phospholipids. In the present study, we take advantage of this technique to analyze the lipids of porcine olfactory mucosa and a membrane fraction enriched in cilia. Thin-layer chromatography (TLC) and (31)P-NMR analyses of the lipid extracts were also performed in parallel. MALDI-TOF-MS allowed the identification of lipid classes in the total lipid extract and individual lipids present in the main TLC bands. The comparison between the composition of the two lipid extracts showed that: (1) cardiolipin, present in small amount in the whole olfactory mucosa lipid extract, was absent in the extract of membranes enriched in olfactory cilia, (2) phosphatidylethanolamine species were less abundant in ciliary than in whole epithelial membranes, (3) sulfoglycosphingolipids were detected in the lipid extract of ciliary membranes, but not in that of epithelial membranes. Our results indicate that the lipid pattern of ciliary membranes is different from that of whole-tissue membranes and suggest that olfactory receptors require a specific lipid environment for their functioning.
基质9-氨基吖啶最近已被引入用于阴离子和阳离子磷脂的基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱分析。在本研究中，我们利用该技术分析猪嗅觉粘膜和富含纤毛的膜组分的脂质。同时还对脂质提取物进行了薄层色谱（TLC）和（31）P-NMR分析。MALDI-TOF-MS能够鉴定总脂质提取物中的脂质类别以及主要TLC条带中存在的单个脂质。两种脂质提取物组成的比较表明：（1）在整个嗅觉粘膜脂质提取物中含量较少的心磷脂，在富含嗅觉纤毛的膜提取物中不存在；（2）磷脂酰乙醇胺种类在纤毛中的丰度低于整个上皮膜中的丰度；（3）在纤毛膜脂质提取物中检测到了硫代糖鞘脂，但在上皮膜脂质提取物中未检测到。我们的结果表明，纤毛膜的脂质模式与全组织膜的脂质模式不同，并表明嗅觉受体的功能需要特定的脂质环境。
