Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC-US, Sevilla, Spain.
Plant Biotechnol J. 2011 Dec;9(9):1049-60. doi: 10.1111/j.1467-7652.2011.00626.x. Epub 2011 Jun 7.
Starch is an important renewable raw material with an increasing number of applications. Several attempts have been made to obtain plants that produce modified versions of starch or higher starch yield. Most of the approaches designed to increase the levels of starch have focused on the increment of the amount of ADP-glucose or ATP available for starch biosynthesis. In this work, we show that the overexpression of starch synthase class IV (SSIV) increases the levels of starch accumulated in the leaves of Arabidopsis by 30%-40%. In addition, SSIV-overexpressing lines display a higher rate of growth. The increase in starch content as a consequence of enhanced SSIV expression is also observed in long-term storage starch organs such as potato tubers. Overexpression of SSIV in potato leads to increased tuber starch content on a dry weight basis and to increased yield of starch production in terms of tons of starch/hectare. These results identify SSIV as one of the regulatory steps involved in the control of the amount of starch accumulated in plastids.
淀粉是一种重要的可再生原料，其应用越来越广泛。人们已经尝试了几种方法来获得能够产生改性淀粉或更高淀粉产量的植物。大多数旨在提高淀粉水平的方法都集中在增加用于淀粉生物合成的 ADP-葡萄糖或 ATP 的量上。在这项工作中，我们表明，淀粉合酶 IV 类（SSIV）的过表达将拟南芥叶片中积累的淀粉水平提高了 30%-40%。此外，SSIV 过表达系表现出更高的生长速度。在长期储存的淀粉器官（如马铃薯块茎）中，由于增强的 SSIV 表达导致淀粉含量增加的情况也被观察到。在马铃薯中过表达 SSIV 会导致干重基础上的块茎淀粉含量增加，并以吨/公顷为单位增加淀粉产量。这些结果表明 SSIV 是参与控制质体中积累的淀粉量的调节步骤之一。
