MDV感染后MD易感鸡系中CD4基因启动子甲基化水平的下调
Down-regulation of promoter methylation level of CD4 gene after MDV infection in MD-susceptible chicken line.
作者信息
Luo Juan, Yu Ying, Zhang Huanmin, Tian Fei, Chang Shuang, Cheng Hans H, Song Jiuzhou
机构信息
Animal & Avian Sciences Department, University of Maryland, College Park, Maryland, 20740, USA.
College of Animal Sciences, China Agricultural University, Haidian, Beijing, 100193, P.R. China.
出版信息
BMC Proc. 2011 Jun 3;5 Suppl 4(Suppl 4):S7. doi: 10.1186/1753-6561-5-S4-S7.
BACKGROUND
Marek's disease virus (MDV) is an oncovirus that induces lymphoid tumors in susceptible chickens, and may affect the epigenetic stability of the CD4 gene. The purpose of this study was to find the effect of MDV infection on DNA methylation status of the CD4 gene differed between MD-resistant (L63) and -susceptible (L72) chicken lines.
METHODS
Chickens from each line were divided into two groups with one group infected by MDV and the other group as uninfected controls. Then, promoter DNA methylation levels of the CD4 gene were measured by Pyrosequencing; and gene expression analysis was performed by quantitative PCR.
RESULTS
Promoter methylation of the CD4 gene was found to be down-regulated in L72 chickens only after MDV infection. The methylation down-regulation of the CD4 promoter is negatively correlated with up-regulation of CD4 gene expression in the L72 spleen at 21 dpi.
CONCLUSIONS
The methylation fluctuation and mRNA expression change of CD4 gene induced by MDV infection suggested a unique epigenetic mechanism existed in MD-susceptible chickens.
背景
马立克氏病病毒（MDV）是一种致瘤病毒，可在易感鸡中诱发淋巴肿瘤，并可能影响CD4基因的表观遗传稳定性。本研究旨在探讨MDV感染对MD抗性（L63）和易感（L72）鸡品系中CD4基因DNA甲基化状态的影响差异。
方法
将每个品系的鸡分为两组，一组感染MDV，另一组作为未感染对照。然后，通过焦磷酸测序法测定CD4基因启动子的DNA甲基化水平；并通过定量PCR进行基因表达分析。
结果
仅在MDV感染后，发现L72鸡中CD4基因的启动子甲基化被下调。在感染后21天，L72脾脏中CD4启动子的甲基化下调与CD4基因表达上调呈负相关。
结论
MDV感染诱导的CD4基因甲基化波动和mRNA表达变化表明，MD易感鸡中存在独特的表观遗传机制。
Zotero 插件