Yan Yiming, Zhang Huihua, Gao Shuang, Zhang Huanmin, Zhang Xinheng, Chen Weiguo, Lin Wencheng, Xie Qingmei
Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.
Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China.
Front Vet Sci. 2021 May 31;8:659840. doi: 10.3389/fvets.2021.659840. eCollection 2021.
Avian leukosis virus subgroup J (ALV-J) is an oncogenic virus that causes serious economic losses in the poultry industry; unfortunately, there is no effective vaccine against ALV-J. DNA methylation plays a crucial role in several biological processes, and an increasing number of diseases have been proven to be related to alterations in DNA methylation. In this study, we screened ALV-J-positive and -negative chickens. Subsequently, we generated and provided the genome-wide gene expression and DNA methylation profiles by MeDIP-seq and RNA-seq of ALV-J-positive and -negative chicken samples; 8,304 differentially methylated regions (DMRs) were identified by MeDIP-seq analysis ( ≤ 0.005) and 515 differentially expressed genes were identified by RNA-seq analysis ( ≤ 0.05). As a result of an integration analysis, we screened six candidate genes to identify ALV-J-negative chickens that possessed differential methylation in the promoter region. Furthermore, TGFB2 played an important role in tumorigenesis and cancer progression, which suggested TGFB2 may be an indicator for identifying ALV-J infections.
禽白血病病毒J亚群（ALV-J）是一种致癌病毒，给家禽业造成严重经济损失；不幸的是，目前尚无针对ALV-J的有效疫苗。DNA甲基化在多个生物学过程中起关键作用，并且越来越多的疾病已被证明与DNA甲基化改变有关。在本研究中，我们筛选了ALV-J阳性和阴性鸡。随后，我们通过对ALV-J阳性和阴性鸡样本进行MeDIP-seq和RNA-seq，生成并提供了全基因组基因表达和DNA甲基化图谱；通过MeDIP-seq分析（≤0.005）鉴定出8304个差异甲基化区域（DMR），通过RNA-seq分析（≤0.05）鉴定出515个差异表达基因。通过整合分析，我们筛选出六个候选基因，以鉴定在启动子区域具有差异甲基化的ALV-J阴性鸡。此外，TGFB2在肿瘤发生和癌症进展中起重要作用，这表明TGFB2可能是鉴定ALV-J感染的一个指标。
