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中国荷斯坦奶牛中与乳腺炎指示性状相关的DNA异常甲基化水平及JAK-STAT信号通路相关基因的关联分析

Association of Aberrant DNA Methylation Level in the and JAK-STAT-Pathway-Related Genes with Mastitis Indicator Traits in Chinese Holstein Dairy Cattle.

作者信息

Usman Tahir, Ali Nawab, Wang Yachun, Yu Ying

机构信息

Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

College of Veterinary Science and Animal Husbandry, Abdul Wali Khan University, Mardan 23200, Pakistan.

出版信息

Animals (Basel). 2021 Dec 29;12(1):65. doi: 10.3390/ani12010065.

DOI:10.3390/ani12010065
PMID:35011171
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8749870/
Abstract

The present study was designed to evaluate the gene expression and DNA methylation level in the promoter region of the and the JAK-STAT-pathway-related genes. A total of 24 samples were deployed in the gene expression and 118 samples were used in the DNA methylation study. Student's -tests were used to analyze the gene expression and DNA methylation. The evaluation of DNA methylation in promoter regions of and revealed hypo-methylation levels of CpG sites and higher gene expression in cows diagnosed with mastitis as compared to the healthy control, and vice versa in those with . DNA methylation was negatively correlated with gene expression in , , and genes. Six, two, and four active transcription factors were identified on the CpG sites in the promoter regions of , , and genes, respectively. Regarding correlation analysis, the DNA methylation levels of showed significantly higher positive correlations with somatic cell counts ( < 0.05). Findings of the current study inferred that aberrant DNA methylation in the CpG sites at the 1 kb promoter region in , , and genes due to mastitis in cows can be used as potential epigenetic markers to estimate bovine mastitis susceptibility in dairy cattle.

摘要

本研究旨在评估[具体基因]启动子区域以及JAK-STAT信号通路相关基因的基因表达和DNA甲基化水平。基因表达研究共使用了24个样本，DNA甲基化研究则使用了118个样本。采用学生t检验分析基因表达和DNA甲基化情况。对[具体基因]启动子区域的DNA甲基化评估显示，与健康对照相比，患乳腺炎奶牛的CpG位点甲基化水平较低，基因表达较高，而患[另一种疾病]的奶牛则相反。[具体基因]、[具体基因]和[具体基因]的DNA甲基化与基因表达呈负相关。在[具体基因]、[具体基因]和[具体基因]启动子区域的CpG位点上分别鉴定出6个、2个和4个活性转录因子。相关性分析表明，[具体基因]的DNA甲基化水平与体细胞计数呈显著正相关（P<0.05）。本研究结果推断，奶牛乳腺炎导致的[具体基因]、[具体基因]和[具体基因]基因1 kb启动子区域CpG位点的异常DNA甲基化可作为潜在的表观遗传标记，用于评估奶牛患乳腺炎的易感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cbb/8749870/80e451e0a577/animals-12-00065-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cbb/8749870/c924b4e8fc1e/animals-12-00065-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cbb/8749870/62e8ad4a6645/animals-12-00065-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cbb/8749870/80e451e0a577/animals-12-00065-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cbb/8749870/c924b4e8fc1e/animals-12-00065-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cbb/8749870/62e8ad4a6645/animals-12-00065-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cbb/8749870/80e451e0a577/animals-12-00065-g003.jpg

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