Lestari Dwi, Murtini Sri, Ulupi Niken, Gunawan Asep, Sumantri Cece
Graduate School of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, Indonesia.
Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, Indonesia.
Vet World. 2023 May;16(5):1161-1164. doi: 10.14202/vetworld.2023.1161-1164. Epub 2023 May 30.
IPB-D2 chickens are selected from IPB-D1 due to their disease-resistance characteristics. One-way to evaluate the strength of a chicken's immune system is by examining the number of circulating T lymphocytes. This assessment can be conducted using a modern analytic method called flow cytometry which relies on monoclonal antibodies to detect the relative proportions of each cell and measure the quality and quantity of biological and physical features of cells, including specific membrane or intracellular glycoprotein markers. Therefore, this study aimed to evaluate the population of lymphocytes, cluster of differentiation (CD)4 and CD8 in IPB-D2 chickens.
Flow cytometry was used to evaluate the population of lymphocytes, CD4, and CD8 in IPB-D2 chickens. The data obtained in this study were analyzed by Minitab, and the mean values were compared using a t-test.
The lymphocytes, CD4, and CD8 populations of IPB-D2 chicken with high Newcastle disease (ND) antibody titers were 65.04%, 10.53%, and 5.47%. Meanwhile, this breed, with low ND antibody titers had lymphocytes CD4 and CD8 population of 57.19%, 8.40%, and 4.11 %. The comparison of CD4 and CD8 populations in chickens with high and low ND antibody titers was 1.92 and 2.04, respectively.
IPB-D2 chickens with high ND antibody titers exhibited increased lymphocyte, CD4, and CD8 cell populations in comparison to those with low ND antibody titers. However, the high ND antibody titer group had a lower CD4/CD8 ratio.
IPB - D2鸡因其抗病特性从IPB - D1中选育而来。评估鸡免疫系统强度的一种方法是检查循环T淋巴细胞的数量。这种评估可使用一种名为流式细胞术的现代分析方法进行，该方法依靠单克隆抗体来检测每个细胞的相对比例，并测量细胞生物学和物理特征的质量与数量，包括特定的膜或细胞内糖蛋白标志物。因此，本研究旨在评估IPB - D2鸡的淋巴细胞、分化簇（CD）4和CD8的群体数量。
采用流式细胞术评估IPB - D2鸡的淋巴细胞、CD4和CD8群体数量。本研究获得的数据用Minitab进行分析，均值采用t检验进行比较。
新城疫（ND）抗体效价高的IPB - D2鸡的淋巴细胞、CD4和CD8群体数量分别为65.04%、10.53%和5.47%。同时，该品种ND抗体效价低的鸡的淋巴细胞、CD4和CD8群体数量分别为57.19%、8.40%和4.11%。ND抗体效价高和低的鸡的CD4和CD8群体数量比较分别为1.92和2.04。
与ND抗体效价低的IPB - D2鸡相比，ND抗体效价高的鸡的淋巴细胞、CD4和CD8细胞群体数量增加。然而，ND抗体效价高的组CD4/CD8比值较低。
