Department of Neurophysiology, Juntendo University School of Medicine, Hongo 2-1-1, Bunkyo-ku, Tokyo 113-8421, Japan.
Neurosci Res. 2011 Sep;71(1):49-62. doi: 10.1016/j.neures.2011.05.013. Epub 2011 May 27.
Reward-induced burst firing of dopaminergic neurons has mainly been studied in the primate midbrain. Voltammetry allows high-speed detection of dopamine release in the projection area. Although voltammetry has revealed presynaptic modulation of dopamine release in the striatum, to date, reward-induced release in awakened brains has been recorded only in rodents. To make such recordings, it is possible to use conventional carbon fibres in monkey brains but the use of these fibres is limited by their physical fragility. In this study, constant-potential amperometry was applied to novel diamond microelectrodes for high-speed detection of dopamine. In primate brains during Pavlovian cue-reward trials, a sharp response to a reward cue was detected in the caudate of Japanese monkeys. Overall, this method allows measurements of monoamine release in specific target areas of large brains, the findings from which will expand the knowledge of reward responses obtained by unit recordings.
奖励诱发的多巴胺能神经元爆发性放电主要在灵长类动物的中脑进行研究。伏安法允许在投射区域内高速检测多巴胺的释放。尽管伏安法已经揭示了纹状体中多巴胺释放的突触前调制，但迄今为止，在清醒大脑中记录到的奖励诱导释放仅在啮齿动物中。为了进行这种记录，可以在猴子大脑中使用传统的碳纤维，但这些纤维的使用受到其物理脆弱性的限制。在这项研究中，恒电位安培法应用于新型金刚石微电极，用于高速检测多巴胺。在灵长类动物进行巴甫洛夫线索-奖励试验期间，在日本猕猴的尾状核中检测到对奖励线索的尖锐反应。总的来说，这种方法允许在大型大脑的特定目标区域测量单胺类物质的释放，其发现将扩展通过单位记录获得的奖励反应的知识。
