Microbiology Division, US Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR 72079, USA.
Food Microbiol. 2011 Sep;28(6):1124-8. doi: 10.1016/j.fm.2011.03.003. Epub 2011 Mar 11.
A total of 39 Salmonella enterica serovar Saintpaul strains from imported seafood, pepper and from environmental and clinical samples were analyzed for the presence of virulence genes, antibiotic resistance, plasmid and plasmid replicon types. Pulsed-field gel electrophoresis (PFGE) fingerprinting using the XbaI restriction enzyme and plasmid profiling were performed to assess genetic diversity. None of the isolates showed resistance to ampicillin, chloramphenicol, gentamicin, kanamycin, streptomycin, sulfisoxazole, and tetracycline. Seventeen virulence genes were screened for by PCR. All strains were positive for 14 genes (spiA, sifA, invA, spaN, sopE, sipB, iroN, msgA, pagC, orgA, prgH, lpfC, sitC, and tolC) and negative for three genes (spvB, pefA, and cdtB). Twelve strains, including six from clinical samples and six from seafood, carried one or more plasmids. Large plasmids, sized greater than 50 kb were detected in one clinical and three food isolates. One plasmid was able to be typed as IncI1 by PCR-based replicon typing. There were 25 distinct PFGE-XbaI patterns, clustered to two groups. Cluster A, with 68.5% similarity mainly consists of clinical isolates, while Cluster C, with 67.6% similarity, mainly consisted of shrimp isolates from India. Our findings indicated the genetic diversity of S. Saintpaul in clinical samples, imported seafood, and the environment and that this serotype possesses several virulent genes and plasmids which can cause salmonellosis.
对来自进口海鲜、胡椒和环境及临床样本的 39 株肠炎沙门氏菌圣塔保罗血清型菌株进行了毒力基因、抗生素耐药性、质粒和质粒复制子类型的分析。采用 XbaI 限制性内切酶进行脉冲场凝胶电泳（PFGE）指纹图谱分析和质粒图谱分析，以评估遗传多样性。分离株均对氨苄西林、氯霉素、庆大霉素、卡那霉素、链霉素、磺胺异恶唑和四环素无耐药性。通过 PCR 筛选了 17 个毒力基因。所有菌株均为 14 个基因（spiA、sifA、invA、spaN、sopE、sipB、iroN、msgA、pagC、orgA、prgH、lpfC、sitC 和 tolC）阳性，3 个基因（spvB、pefA 和 cdtB）阴性。包括 6 株来自临床样本和 6 株来自海鲜的 12 株菌株携带一个或多个质粒。在一个临床分离株和三个食品分离株中检测到大小大于 50kb 的大质粒。通过基于 PCR 的复制子分型，发现了一个可被归类为 IncI1 的质粒。PFGE-XbaI 有 25 种不同的模式，聚类为两组。A 组，相似度为 68.5%，主要由临床分离株组成，而 C 组，相似度为 67.6%，主要由来自印度的虾分离株组成。我们的研究结果表明，圣塔保罗血清型沙门氏菌在临床样本、进口海鲜和环境中的遗传多样性，并且该血清型具有多个毒力基因和质粒，可能导致沙门氏菌病。
