Elbashir Salah M, Adnan Adib M, Bowers John, DePaola Angelo, Jahncke Michael, Punchihewage-Don Anuradha J, Da Silva Ligia V, Hashem Fawzy, Parveen Salina
School of Agricultural and Natural Sciences, University of Maryland Eastern Shore, Princess Anne, MD 21853, USA.
College of Computer, Mathematical and Natural Sciences, University of Maryland, College Park, MD 20742, USA.
Pathogens. 2023 Jun 30;12(7):897. doi: 10.3390/pathogens12070897.
The quantity of seafood imported and produced by domestic aquaculture farming has increased. Recently, it has been reported that multidrug-resistant (MDR) Typhimurium may be associated with seafood. However, information is limited to the antimicrobial resistance, virulence properties, and genetic diversity of recovered from imported and domestic seafood. This study investigated the antimicrobial resistance, virulence properties, and genetic diversity of isolated from domestic and imported catfish, shrimp, and tilapia. A total of 127 isolates were tested for the presence of multidrug-resistance (MDR), virulence genes (A, C, C, R), and genetic diversity using the Sensititre micro-broth dilution method, PCR, and pulsed-field gel electrophoresis (PFGE), respectively. All isolates were uniformly susceptible to six (amoxicillin/clavulanic acid, ceftiofur, ceftriaxone, imipenem, nitrofurantoin, and trimethoprim/sulfamethoxazole) of the 17 tested antimicrobials and genetically diverse. Fifty-three percent of the isolates were resistant to at least one antimicrobial and 49% were multidrug resistant. Ninety-five percent of the isolates possessed the A gene, 67% C, and 43% for both C, and R. The results suggest that recovered from seafood is frequently MDR, virulent, and have the ability to cause salmonellosis.
国内水产养殖进口和生产的海产品数量有所增加。最近，有报道称耐多药鼠伤寒沙门氏菌可能与海产品有关。然而，关于从进口和国产海产品中分离出的该菌的抗菌药物耐药性、毒力特性和遗传多样性的信息有限。本研究调查了从国产和进口鲶鱼、虾及罗非鱼中分离出的该菌的抗菌药物耐药性、毒力特性和遗传多样性。分别使用Sensititre微量肉汤稀释法、聚合酶链反应（PCR）和脉冲场凝胶电泳（PFGE）对总共127株分离株进行了耐多药（MDR）、毒力基因（A、C、C、R）和遗传多样性检测。所有分离株对所检测的17种抗菌药物中的六种（阿莫西林/克拉维酸、头孢噻呋、头孢曲松、亚胺培南、呋喃妥因和甲氧苄啶/磺胺甲恶唑）均表现出一致的敏感性，且具有遗传多样性。53%的分离株对至少一种抗菌药物耐药，49%为耐多药。95%的分离株携带A基因，67%携带C基因，43%同时携带C和R基因。结果表明，从海产品中分离出的该菌通常具有耐多药、有毒力且有能力引发沙门氏菌病。
