Gilbert D M, Cohen S N
Department of Genetics, Stanford University School of Medicine, California 94305.
Mol Cell Biol. 1990 Aug;10(8):4345-55. doi: 10.1128/mcb.10.8.4345-4355.1990.
Simian virus 40 (SV40) DNA molecules chromosomally integrated at different sites in three Chinese hamster lung fibroblast lines replicated during the middle portion of S phase but not precisely at the same time in all three cell lines. The time of replication was unrelated to the presence of T antigen or to its relative activity in promoting SV40 replication. SV40 sequences and chromosomal DNA sequences adjacent to the SV40 insert in one cell line expressing a temperature-sensitive T antigen showed a T-antigen-independent difference in replication timing from the homologous, allelic locus not linked to SV40. Our results indicate that the timing of replication of these integrated SV40 molecules is dependent upon the site of integration and is not determined by the level of T antigen replication-promoting activity.
猿猴病毒40（SV40）的DNA分子整合到三种中国仓鼠肺成纤维细胞系的不同染色体位点上，在S期的中期进行复制，但并非在所有三种细胞系中都精确地在同一时间复制。复制时间与T抗原的存在或其促进SV40复制的相对活性无关。在一个表达温度敏感型T抗原的细胞系中，与SV40插入片段相邻的SV40序列和染色体DNA序列，与未与SV40连锁的同源等位基因座相比，在复制时间上表现出与T抗原无关的差异。我们的结果表明，这些整合的SV40分子的复制时间取决于整合位点，而不是由T抗原复制促进活性的水平决定的。
