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An insulin response element in the glyceraldehyde-3-phosphate dehydrogenase gene binds a nuclear protein induced by insulin in cultured cells and by nutritional manipulations in vivo.

作者信息

Nasrin N, Ercolani L, Denaro M, Kong X F, Kang I, Alexander M

机构信息

Howard Hughes Medical Institute, Massachusetts General Hospital, Harvard Medical School, Boston 02114.

出版信息

Proc Natl Acad Sci U S A. 1990 Jul;87(14):5273-7. doi: 10.1073/pnas.87.14.5273.

DOI:10.1073/pnas.87.14.5273
PMID:2164673
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC54305/
Abstract

Two independent cis-acting insulin response elements (IREs) in the gene encoding glyceraldehyde-3-phosphate dehydrogenase [D-glyceraldehyde-3-phosphate: NAD+ oxidoreductase (phosphorylating), EC 1.2.1.12], designated IRE-A and IRE-B, are sufficient to direct insulin-inducible gene expression. Using the electrophoretic mobility shift assay, a 4-fold increase in the amount of IRE-A DNA bound to nuclear proteins was detected when extracts isolated from insulin-stimulated differentiated 3T3-L1 cells or from the liver of rats refed a high-carbohydrate/low-fat diet after a 72-hr fast were compared to control nuclear extracts. The points of contact between protein and IRE-A DNA may represent a sequence recognized by at least one class of insulin-sensitive transcription factor(s).

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fc5/54305/442b0c9e4732/pnas01039-0052-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fc5/54305/c3918b883dd0/pnas01039-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fc5/54305/1166e71b687e/pnas01039-0051-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fc5/54305/66ad60f607f8/pnas01039-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fc5/54305/442b0c9e4732/pnas01039-0052-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fc5/54305/c3918b883dd0/pnas01039-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fc5/54305/1166e71b687e/pnas01039-0051-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fc5/54305/66ad60f607f8/pnas01039-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fc5/54305/442b0c9e4732/pnas01039-0052-b.jpg

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Mutation of the c-fos gene dyad symmetry element inhibits serum inducibility of transcription in vivo and the nuclear regulatory factor binding in vitro.
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己糖激酶 2 相关糖酵解过载和非计划性糖酵解——胰岛素抵抗和糖尿病血管并发症发展的驱动因素。
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