DiPaolo J A, Woodworth C D, Popescu N C, Koval D L, Lopez J V, Doniger J
Laboratory of Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Virology. 1990 Aug;177(2):777-9. doi: 10.1016/0042-6822(90)90548-6.
A subgenomic region of HSV-2, BglII N, is capable of converting immortal genital epithelial cells containing integrated HPV16 sequences into tumorigenic squamous cell carcinoma cells. Moreover, tumor-derived cultured cells and immortal cells that had been transfected with HSV-2/Bg/II N and kept in continuous culture subsequently lost the HSV-2 sequences. The HSV-2/Bg/II N sequence was ineffective on normal cells. Thus, HSV-2/Bg/II N may act as a cofactor in the genesis of a carcinoma but is not required to maintain the transformed phenotype. Although papillomaviruses (HPVs) are currently receiving much attention because of their association with cervical squamous carcinomas, ample reasons exist to suggest a multifactorial etiology in which additional factors are necessary to convert dysplastic lesions to carcinomas. The hypothesis that specific HPV types may be necessary but not sufficient to cause cancer is reinforced. Thus, HSV-2-transforming sequences have a potential role in the etiology of human cervical cancer.
单纯疱疹病毒2型（HSV - 2）的一个亚基因组区域BglII N，能够将含有整合型人乳头瘤病毒16型（HPV16）序列的永生化生殖器上皮细胞转化为致瘤性鳞状细胞癌细胞。此外，用HSV - 2/Bg/II N转染并持续培养的肿瘤来源的培养细胞和永生化细胞随后丢失了HSV - 2序列。HSV - 2/Bg/II N序列对正常细胞无效。因此，HSV - 2/Bg/II N可能在癌发生过程中作为一种辅助因子，但维持转化表型并不需要它。尽管乳头瘤病毒（HPV）目前因与宫颈鳞状细胞癌相关而备受关注，但有充分理由表明其病因是多因素的，其中还需要其他因素才能将发育异常病变转化为癌症。特定HPV类型可能是导致癌症所必需但不充分的这一假说得到了加强。因此，HSV - 2转化序列在人类宫颈癌病因学中具有潜在作用。
