Manservigi R, Cassai E, Deiss L P, Di Luca D, Segala V, Frenkel N
Virology. 1986 Nov;155(1):192-201. doi: 10.1016/0042-6822(86)90179-0.
Ten human genital invasive squamous cell carcinomas and five human premalignant tissues were analyzed for the presence of selected sets of herpes simplex virus 2 (HSV-2) DNA sequences. Two vulvar tumors and one vulvar dysplastic tissue were found to contain DNA sequences homologous to the BglII O fragment (coordinates 0.38-0.42) and the BglII N fragment (coordinates 0.58-0.63) of HSV-2 DNA. These two fragments overlap the subsets of HSV-1 and HSV-2 DNA sequences (respectively) shown previously to transform cells in culture. Sequences homologous to an additional HSV-2 DNA probe (BglII G) were not detected in the same tumors. Surprisingly, in each of the two positive vulvar tumors, the BglII N and BglII O sequences appeared to be linked, whereas in the standard HSV-2 genome the two fragments are separated by approximately 26 kb. This finding suggested that the two sets of sequences may have rearranged prior to or following the association of the HSV DNA sequences with the tumor cells. The same set of 10 tumors were analyzed for the presence of sequences complementary to human papillomavirus 16 (HPV16) DNA. The HPV16 DNA probe hybridized to three of six cervical tumors, whereas no hybridization was detected with the two vulvar tumors which contained the HSV DNA sequences.
对10例人类生殖器浸润性鳞状细胞癌和5例人类癌前组织进行分析，以检测特定的单纯疱疹病毒2型（HSV-2）DNA序列。发现两例外阴肿瘤和一例外阴发育异常组织含有与HSV-2 DNA的BglII O片段（坐标0.38 - 0.42）和BglII N片段（坐标0.58 - 0.63）同源的DNA序列。这两个片段分别与先前显示可在培养中转化细胞的HSV-1和HSV-2 DNA序列子集重叠。在相同肿瘤中未检测到与另一个HSV-2 DNA探针（BglII G）同源的序列。令人惊讶的是，在两例阳性外阴肿瘤中，BglII N和BglII O序列似乎是相连的，而在标准HSV-2基因组中，这两个片段被大约26 kb的距离隔开。这一发现表明，这两组序列可能在HSV DNA序列与肿瘤细胞关联之前或之后发生了重排。对同一组10例肿瘤分析了与人乳头瘤病毒16型（HPV16）DNA互补的序列的存在情况。HPV16 DNA探针与6例宫颈肿瘤中的3例杂交，而在含有HSV DNA序列的两例外阴肿瘤中未检测到杂交信号。
