Bakır Ayfer, Yüzügüldü Betül, Tanık Eylül Beren, Kürkçü Muhammed Furkan, Korkut Gizem, Duran Firdevs Şahin
Department of Microbiology, Ankara Etlik City Hospital, University of Health Sciences, Ankara 06170, Türkiye.
Department of Pathology, Ankara Etlik City Hospital, University of Health Sciences, Ankara 06170, Türkiye.
Trop Med Infect Dis. 2025 Jul 17;10(7):200. doi: 10.3390/tropicalmed10070200.
Persistent high-risk human papillomavirus (HR-HPV) infection is closely associated with the development of cervical intraepithelial neoplasia (CIN) and cervical cancer. In recent years, the potential impact of viral co-infections on this process has also been investigated. This study investigated the presence of HR-HPV, HSV-1/2, and HHV-8 DNA in formalin-fixed paraffin-embedded (FFPE) cervical biopsy samples, as well as their association with lesion severity. A total of 276 FFPE cervical tissue samples were evaluated. Viral DNA was detected by real-time PCR. The samples were histopathologically classified as normal/non-dysplastic, low-grade (LSIL), and high-grade (HSIL) lesions. HR-HPV DNA was detected in 112 samples (40.6%), with the highest prevalence observed in the 30-39 age group (51.2%). Among the HPV-positive cases, 46.5% (52/112) had single-type infections, 32.1% (36/112) had multiple-type infections, and 21.4% (24/112) were untypable. Together, these categories accounted for all HPV-positive samples. The most common genotype was HPV-16 (16.7%). HHV-8 and HSV-2 DNA were not detected. HSV-1 DNA was detected in only three non-dysplastic, HPV-negative cervical samples. In conclusion, HR-HPV DNA was detected in 40.6% of cervical biopsy samples and showed a significant association with increasing histological severity, highlighting its critical role in the progression of cervical lesions. Although the absence of HHV-8 and HSV-2 suggests a limited contribution of these viruses to cervical disease, the use of a single real-time PCR assay limits the ability to draw generalized conclusions regarding their clinical relevance. Further large-scale, multicenter studies employing both tissue-based and serological approaches are needed to validate these findings and to better understand the dynamics of viral co-infections in cervical disease.
持续性高危型人乳头瘤病毒（HR-HPV）感染与宫颈上皮内瘤变（CIN）及宫颈癌的发生密切相关。近年来，病毒合并感染对这一过程的潜在影响也受到了研究。本研究调查了福尔马林固定石蜡包埋（FFPE）宫颈活检样本中HR-HPV、单纯疱疹病毒1/2型（HSV-1/2）和卡波西肉瘤相关疱疹病毒（HHV-8）DNA的存在情况，以及它们与病变严重程度的关联。共评估了276份FFPE宫颈组织样本。通过实时聚合酶链反应（PCR）检测病毒DNA。样本经组织病理学分类为正常/非发育异常、低级别（LSIL）和高级别（HSIL）病变。112份样本（40.6%）检测到HR-HPV DNA，其中30-39岁年龄组的患病率最高（51.2%）。在HPV阳性病例中，46.5%（52/112）为单型感染，32.1%（36/112）为多型感染，21.4%（24/112）无法分型。这些类别共同构成了所有HPV阳性样本。最常见的基因型是HPV-16（16.7%）。未检测到HHV-8和HSV-2 DNA。仅在3份非发育异常、HPV阴性的宫颈样本中检测到HSV-1 DNA。总之，40.6%的宫颈活检样本中检测到HR-HPV DNA，且与组织学严重程度增加显著相关，突出了其在宫颈病变进展中的关键作用。虽然未检测到HHV-8和HSV-2表明这些病毒对宫颈疾病的贡献有限，但使用单一实时PCR检测方法限制了就其临床相关性得出普遍结论的能力。需要进一步开展大规模、多中心研究，采用基于组织和血清学的方法来验证这些发现，并更好地了解宫颈疾病中病毒合并感染的动态情况。
