Defaveri J, Graybill J R
Veterans Administration Hospital Audie L. Murphy, Merton Minter, San Antonio, Texas.
Am J Trop Med Hyg. 1990 Jun;42(6):601-6. doi: 10.4269/ajtmh.1990.42.601.
Congenitally athymic (nu/nu) mice were inoculated sc with 10(5) conidia of Fonsecaea pedrosoi and treated orally from the 1st to the 16th week of infection with either a new triazole, SCH39304, or itraconazole at doses of 20 or 60 mg/kg/day. The volumes of the lesions were measured with calipers at 4 week intervals and compared statistically by the Wilcoxon test. At the end of the experiment, mice were killed and samples of the lesions were examined histopathologically and by electron microscopy. Treatment with itraconazole or SCH39304 significantly reduced lesion sizes as compared with controls. There were no differences between the 2 drugs at the dosages used. Histopathologically, lesions of mice treated with either drug had less inflammation with fewer fungi and more diffuse fibrosis than controls. Electron microscopy showed damage to the fungal cell walls in mice treated with itraconazole or SCH39304, characterized by gaps, fragmentation, and delamination. These studies confirm clinical observations that itraconazole is effective in chromoblastomycosis and suggest that SCH39304 should be considered for clinical evaluation.
将先天性无胸腺（nu/nu）小鼠皮下接种10⁵ 个裴氏着色真菌分生孢子，并在感染的第1周至第16周口服给予新的三唑类药物SCH39304或伊曲康唑，剂量为20或60毫克/千克/天。每隔4周用卡尺测量病变体积，并通过Wilcoxon检验进行统计学比较。实验结束时，处死小鼠，对病变样本进行组织病理学检查和电子显微镜检查。与对照组相比，伊曲康唑或SCH39304治疗显著减小了病变大小。在所使用的剂量下，两种药物之间没有差异。组织病理学上，用任何一种药物治疗的小鼠病变炎症较轻，真菌较少，纤维化更弥漫。电子显微镜显示，用伊曲康唑或SCH39304治疗的小鼠真菌细胞壁受损，特征为出现间隙、碎片化和分层。这些研究证实了伊曲康唑治疗着色芽生菌病有效的临床观察结果，并表明SCH39304应考虑进行临床评估。
