Flicker T M, Green S A
Chemistry Department, Michigan Technological University, Houghton, Michigan 49931-1295.
Anal Chem. 1998 May 1;70(9):2008-12. doi: 10.1021/ac970858f.
Carbon-centered radicals were trapped from gas-phase cigarette smoke and diesel engine exhaust by reaction with a nitroxide, 3-amino-2,2,5,5-tetramethyl-1-pyrrolidinyloxy (3AP). The resulting mixture of stable, diamagnetic adducts was derivatized with naphthalenedicarboxaldehyde (NDA) to produce highly fluorescent products. Derivatives were separated by high-performance liquid chromatography (HPLC), which revealed distinctly different suites of radicals present in the two systems. Integration of HPLC peaks gave approximately 22 ± 7 nmol of radicals per cigarette and 3 ± 1 nmol of radicals per liter of diesel engine exhaust. An estimated 8-10 different carbon-centered radical species are present in each system.
通过与氮氧化物3-氨基-2,2,5,5-四甲基-1-吡咯烷氧基（3AP）反应，从气相香烟烟雾和柴油发动机尾气中捕获以碳为中心的自由基。所得稳定的抗磁性加合物混合物用萘二甲醛（NDA）衍生化，以产生高荧光产物。通过高效液相色谱（HPLC）分离衍生物，结果显示两个系统中存在明显不同的自由基组合。HPLC峰的积分结果表明，每支香烟中约有22±7 nmol的自由基，每升柴油发动机尾气中有3±1 nmol的自由基。每个系统中估计存在8-10种不同的以碳为中心的自由基物种。
