Bartalis Judit, Chan W Geoffrey, Wooten Jan B
Philip Morris USA Postgraduate Research Program, P.O. Box 26583, Richmond, Virginia 23261, USA.
Anal Chem. 2007 Jul 1;79(13):5103-6. doi: 10.1021/ac070561+. Epub 2007 May 26.
Radicals in cigarette smoke have been proposed to contribute to the harm caused by cigarette smoking. For the first time, using HPLC and high-resolution mass spectrometry analysis of stable radical adducts, we have identified specific radical species in cigarette smoke: 7 acyl and 11 alkylaminocarbonyl radicals. Their combined abundance measured in fresh whole smoke from a single 2R4F cigarette is approximately 225 nmol (1.4 x 10(17) radicals). The fiberglass Cambridge filter pad conventionally employed to separate the gas phase from mainstream smoke was found to reduce the apparent yield of these radicals, introducing artifacts of measurement. The long-accepted steady-state mechanism for the formation of carbon-centered radicals in cigarette smoke involving NO2 chemistry cannot account for these newly identified radicals, and it does not in general appear to be a major source of carbon-centered radicals in fresh mainstream cigarette smoke. Consequently, we suggest that the precise nature of radicals in cigarette smoke warrants reexamination.
香烟烟雾中的自由基被认为是导致吸烟危害的原因之一。我们首次使用高效液相色谱法（HPLC）和高分辨率质谱分析法对稳定自由基加合物进行分析，从而确定了香烟烟雾中的特定自由基种类：7种酰基自由基和11种烷基氨基羰基自由基。在一支2R4F香烟产生的新鲜全烟雾中测得的这些自由基的总丰度约为225纳摩尔（1.4×10¹⁷个自由基）。人们发现，传统上用于从主流烟雾中分离气相的玻璃纤维剑桥滤片会降低这些自由基的表观产率，从而引入测量误差。长期以来被认可的、涉及二氧化氮化学过程的香烟烟雾中碳中心自由基形成的稳态机制无法解释这些新发现的自由基，而且总体而言，它似乎并不是新鲜主流香烟烟雾中碳中心自由基的主要来源。因此，我们认为香烟烟雾中自由基的精确性质值得重新审视。
