Flicker T M, Green S A
Department of Chemistry, Michigan Technological University, Houghton, Michigan 49931, USA.
Environ Health Perspect. 2001 Aug;109(8):765-71. doi: 10.1289/ehp.01109765.
We used an improved method for trapping carbon-centered radicals (.R) from the gas-phase to compare radical suites trapped from various tobacco smoke and model smoke systems. Using a nitroxide trap, 3-amino-2,2,5,5-tetramethyl-1-pyrrolidinyloxy (3AP), on solid support, we trapped radicals directly from the gas phase, washed them off the support, and analyzed them with HPLC. Separation of the trapped radicals showed that each tobacco type produced a unique radical suite of 4-10 distinct peaks. Gas mixtures used to model tobacco smoke consisted of nitric oxide, air, isoprene, and methanol. The model systems produced radical suites of four major and several minor peaks, two of which matched peaks in tobacco smoke chromatograms. Quantities of radicals trapped from tobacco smoke were: 54 +/- 2 nmol .R per Marlboro cigarette, 66 +/- 9 nmol .R per Djarum clove cigarette, and 185 +/- 9 nmol .R per Swisher Sweet cigar. In these experiments oxygen competes with the nitroxide trap for gas-phase radicals. A kinetic analysis of the O2 competition shows that actual radical concentrations in the smoke were approximately 100-fold higher than measured.
我们采用了一种改进的方法来捕获气相中的碳中心自由基（·R），以比较从各种烟草烟雾和模拟烟雾系统中捕获的自由基组合。使用固定在固体载体上的氮氧化物捕集剂3-氨基-2,2,5,5-四甲基-1-吡咯烷氧基（3AP），我们直接从气相中捕获自由基，将其从载体上洗脱下来，并用高效液相色谱法进行分析。对捕获的自由基进行分离后发现，每种烟草类型都会产生一个独特的自由基组合，包含4至10个不同的峰。用于模拟烟草烟雾的气体混合物由一氧化氮、空气、异戊二烯和甲醇组成。模拟系统产生的自由基组合有四个主要峰和几个次要峰，其中两个与烟草烟雾色谱图中的峰相匹配。从烟草烟雾中捕获的自由基数量为：每支万宝路香烟54±2 nmol·R，每支丁香丁香烟66±9 nmol·R，每支斯威舍甜味雪茄185±9 nmol·R。在这些实验中，氧气与氮氧化物捕集剂竞争气相中的自由基。对氧气竞争的动力学分析表明，烟雾中实际的自由基浓度比测量值高约100倍。
