Department of Animal Genetics, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn, Poland.
Meat Sci. 2011 Dec;89(4):440-3. doi: 10.1016/j.meatsci.2011.05.008. Epub 2011 May 19.
The aim of the study was to check whether different genotypes at acyl-CoA synthetase (ACSL4 locus, SNP G2645A) are associated with pork quality. 132 (Landrace × Yorkshire) × Duroc fatteners were genotyped by originally developed PCR-RFLP method. Upon the slaughter, the samples of longissimus lumborum muscle were taken from each carcass to determine the following parameters: content of water, protein and fat, pH (45 min, 24, 48, 96, and 144 h post mortem), electrical conductivity, drip loss, meat lightness, glycolytic potential, glycogen and lactate contents in meat. Among several associations observed, the highly significant (p<0.01) was found for intramuscular fat (IMF) content. Pigs with genotype GG revealed the highest content of IMF - 2.47%.
本研究旨在检查酰基辅酶 A 合成酶（ACSL4 基因座，SNP G2645A）的不同基因型是否与猪肉品质有关。通过最初开发的 PCR-RFLP 方法对 132 头（长白猪×约克夏）×杜洛克育肥猪进行基因分型。屠宰后，从每头胴体上采集背最长肌肌肉样本，以确定以下参数：水分、蛋白质和脂肪含量、pH 值（宰后 45 分钟、24、48、96 和 144 小时）、电导率、滴水损失、肉色、糖酵解潜力、肉中糖原和乳酸含量。在观察到的几种关联中，发现肌内脂肪（IMF）含量存在高度显著差异（p<0.01）。基因型为 GG 的猪表现出最高的 IMF 含量 - 2.47%。
