Ye Manhong, Zhou Bin, Wei Shanshan, Ding MengMeng, Lu Xinghui, Shi Xuehao, Ding Jiatong, Yang Shengmei, Wei Wanhong
College of Bioscience and Biotechnology, Yangzhou University, Jiangsu Province, 225009, China.
College of Animal Science and Technology, Yangzhou University, Jiangsu Province, 225009, China
G3 (Bethesda). 2016 Jul 7;6(7):2081-90. doi: 10.1534/g3.116.029793.
Despite the fact that squab is consumed throughout the world because of its high nutritional value and appreciated sensory attributes, aspects related to its characterization, and in particular genetic issues, have rarely been studied. In this study, meat traits in terms of pH, water-holding capacity, intramuscular fat content, and fatty acid profile of the breast muscle of squabs from two meat pigeon breeds were determined. Breed-specific differences were detected in fat-related traits of intramuscular fat content and fatty acid composition. RNA-Sequencing was applied to compare the transcriptomes of muscle and liver tissues between squabs of two breeds to identify candidate genes associated with the differences in the capacity of fat deposition. A total of 27 differentially expressed genes assigned to pathways of lipid metabolism were identified, of which, six genes belonged to the peroxisome proliferator-activated receptor signaling pathway along with four other genes. Our results confirmed in part previous reports in livestock and provided also a number of genes which had not been related to fat deposition so far. These genes can serve as a basis for further investigations to screen markers closely associated with intramuscular fat content and fatty acid composition in squabs. The data from this study were deposited in the National Center for Biotechnology Information (NCBI)'s Sequence Read Archive under the accession numbers SRX1680021 and SRX1680022. This is the first transcriptome analysis of the muscle and liver tissue in Columba using next generation sequencing technology. Data provided here are of potential value to dissect functional genes influencing fat deposition in squabs.
尽管乳鸽因其高营养价值和良好的感官特性而在全球范围内被食用，但其特征方面，特别是遗传问题，却很少被研究。在本研究中，测定了两个肉鸽品种乳鸽胸肌的pH值、持水能力、肌内脂肪含量和脂肪酸谱等肉品质性状。在肌内脂肪含量和脂肪酸组成等与脂肪相关的性状上检测到了品种特异性差异。应用RNA测序技术比较两个品种乳鸽肌肉和肝脏组织的转录组，以鉴定与脂肪沉积能力差异相关的候选基因。共鉴定出27个参与脂质代谢途径的差异表达基因，其中6个基因属于过氧化物酶体增殖物激活受体信号通路，还有其他4个基因。我们的结果部分证实了之前在牲畜方面的报道，同时也提供了一些迄今为止与脂肪沉积无关的基因。这些基因可作为进一步研究的基础，以筛选与乳鸽肌内脂肪含量和脂肪酸组成密切相关的标记。本研究的数据已存入美国国立生物技术信息中心（NCBI）的序列读数存档库，登录号为SRX1680021和SRX1680022。这是首次使用下一代测序技术对鸽科动物的肌肉和肝脏组织进行转录组分析。这里提供的数据对于剖析影响乳鸽脂肪沉积的功能基因具有潜在价值。
