Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón II, 4to piso, Departamento de Ecología, Genética y Evolución, Lab. 62, C1428EHA, Buenos Aires, Argentina;
Am J Bot. 2005 Feb;92(2):352-69. doi: 10.3732/ajb.92.2.352.
In order to clarify the relationships among southern South American (sSA) representatives of the genus Ilex, an amplified fragment length polymorphism (AFLP) analysis was accomplished. In addition, the phylogenetic relationships of the species were studied using ribosomal internal transcribed spacer (ITS) sequence data alone and in combination with AFLP data, taking into account the possible existence of paralogous sequences and the influence of alignment parameters. To explore stability of phylogenetic hypotheses, a sensitivity analysis was performed using 15 indel-substitution models. Within each species assayed, the AFLPs allowed the recognition of several diagnostic bands. Furthermore, the AFLP analysis revealed that individuals belonging to the same morpho-species formed coherent clades. In addition, some cases of geographical association were noted. Studies on ITS sequences revealed divergence between data obtained herein and sequence data downloaded from GenBank. The sensitivity analyses yielded different interspecific hypotheses of relationships. Notwithstanding, analyses of the ITS data alone and in combination with AFLPs, rendered clades stable to variation in the analytical parameters. Topologies obtained for the AFLPs, the ITS data alone and the combined analyses, demonstrated the existence of a group formed by I. argentina, I. brasiliensis, I. brevicuspis, I. integerrima, and I. theezans, and that I. dumosa and I. paraguariensis were distantly related to the former. Incongruence with traditional taxonomical treatments was found.
为了阐明南美的冬青属（Ilex）代表种之间的关系，进行了扩增片段长度多态性（AFLP）分析。此外，还单独使用核糖体内转录间隔区（ITS）序列数据以及将其与 AFLP 数据相结合，研究了物种的系统发育关系，同时考虑了可能存在的旁系序列和比对参数的影响。为了探索系统发育假说的稳定性，使用了 15 种插入/缺失替代模型进行了敏感性分析。在所测试的每个种内，AFLP 允许识别出几个诊断带。此外，AFLP 分析表明属于同一形态种的个体形成了一致的分支。此外，还注意到了一些地理关联的情况。对 ITS 序列的研究揭示了此处获得的数据与从 GenBank 下载的序列数据之间的差异。敏感性分析产生了不同的种间关系假设。尽管如此，仅对 ITS 数据以及与 AFLP 的联合分析进行分析，使得分支在分析参数的变化下保持稳定。AFLP、ITS 数据单独以及联合分析获得的拓扑结构表明，存在一个由 I. argentina、I. brasiliensis、I. brevicuspis、I. integerrima 和 I. theezans 组成的群组，而 I. dumosa 和 I. paraguariensis 与前者关系较远。发现与传统分类处理不一致。
