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夏威夷特有掌状叶蒲葵属植物(棕榈科)微卫星的开发及其在同属植物中的应用。

Development of microsatellites in the Hawaiian endemic palm Pritchardia martii (Arecaceae) and their utility in congeners.

机构信息

Department of Biology, Colorado State University, Fort Collins, Colorado 80523-1878, USA.

出版信息

Am J Bot. 2011 Jun;98(6):e139-40. doi: 10.3732/ajb.1100073. Epub 2011 May 27.

DOI:10.3732/ajb.1100073
PMID:21653501
Abstract

PREMISE OF THE STUDY

Pritchardia (loulu palm) is the seventh largest flowering plant genus in the Hawaiian archipelago, and many species are of high conservation concern. The island radiation has produced many cryptic species complexes across fine ecological gradients. Microsatellite primers were optimized to investigate genetic diversity of Pritchardia martii (Gaudich.) H. Wendl. and species boundaries among the Hawaiian Pritchardia.

METHODS AND RESULTS

Six new loci and three previously described loci for a closely related genus, Phoenix, were optimized and tested. Five loci were polymorphic, and 72 alleles were detected across loci within P. martii. The five loci were also polymorphic across the other 27 currently recognized Pritchardia species. •

CONCLUSIONS

These results indicate the utility of these microsatellite markers for understanding the conservation genetics of P. martii and species delimitation and hybridization across the genus.

摘要

研究前提

蒲桃叶藤属(loulu 棕榈)是夏威夷群岛中第七大开花植物属,许多物种都受到高度保护关注。岛屿辐射产生了许多在精细生态梯度上的隐种复合体。微卫星引物被优化用于研究夏威夷蒲桃叶藤属的遗传多样性和种间界限。

方法和结果

为密切相关的属凤凰木优化和测试了六个新的位点和三个之前描述的位点。五个位点是多态的,在 P. martii 内的各个位点检测到 72 个等位基因。这五个位点在其他 27 个目前公认的蒲桃叶藤属物种中也是多态的。

结论

这些结果表明这些微卫星标记对于理解 P. martii 的保护遗传学以及种间界限和杂交具有实用价值。

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引用本文的文献

1
Evaluating multiple criteria for species delimitation: an empirical example using Hawaiian palms (Arecaceae: Pritchardia).评估物种界定的多个标准:以夏威夷掌类植物(棕榈科:蒲葵属)为例的实证研究。
BMC Evol Biol. 2012 Feb 22;12:23. doi: 10.1186/1471-2148-12-23.