Menezes E V, Souto W F S, Ciampi A Y, Azevedo V C R, Valério H M, Pimenta M A S
Laboratório de Genética da Conservação, Universidade Estadual de Montes Claros, Campus Universitário Professor Darcy Ribeiro, Montes Claros, MG, Brasil.
Genet Mol Res. 2012 Nov 28;11(4):4058-62. doi: 10.4238/2012.September.17.6.
Mauritia flexuosa L. (Arecaceae) is a palm tree species known as buriti that occurs in the Cerrado biome. It is characteristic of the vereda, a typical ecosystem of central Brazil. In this phytophysiognomy, M. flexuosa and other groups of arboreal-herbaceous species develop in open fields with very humid soils. M. flexuosa can be found in forest borders and is a palm tree with a wide distribution in South America (Brazil, Colombia, Venezuela, French Guyana Ecuador, Peru, and Bolivia). The main objectives of this study were to develop simple sequence repeat marker-enriched libraries and to characterize these loci in buriti palm to facilitate future population studies. A total of 40 sequences derived from the microsatellite-enriched libraries were selected for primer design. The optimization results showed that 9 primer pairs could successfully amplify polymorphic target fragments of the expected sizes. The data also show that the described primers can be used in population genetic studies in M. flexuosa to obtain information that will inform conservation and management strategies.
毛里求斯棕（Mauritia flexuosa L.，棕榈科）是一种名为布里蒂棕榈的棕榈树种，生长于塞拉多生物群落。它是巴西中部典型生态系统——湿地的特征物种。在这种植物群落中，毛里求斯棕和其他乔木 - 草本物种群生长在土壤非常湿润的开阔地带。毛里求斯棕可在森林边缘找到，是一种在南美洲（巴西、哥伦比亚、委内瑞拉、法属圭亚那、厄瓜多尔、秘鲁和玻利维亚）广泛分布的棕榈树。本研究的主要目的是构建简单序列重复标记富集文库，并对布里蒂棕榈中的这些位点进行表征，以促进未来的种群研究。总共从微卫星富集文库中选取了40个序列用于引物设计。优化结果表明，9对引物能够成功扩增出预期大小的多态性目标片段。数据还表明，所描述的引物可用于毛里求斯棕的种群遗传学研究，以获取有助于保护和管理策略的信息。
