Kataoka Yu, Tamaki Yukimichi, Miyazaki Takashi
Department of Oral Biomaterials and Technology, School of Dentistry, Showa University, Tokyo, Japan.
Biomed Mater Eng. 2011;21(2):113-21. doi: 10.3233/BME-2011-0661.
Wire-type electric discharge machining has been applied to the manufacture of endosseous titanium implants as this computer associated technique allows extremely accurate complex sample shaping with an optimal micro textured surface during the processing. Since the titanium oxide layer is sensitively altered by each processing, the authors hypothesized that this technique also up-regulates biological responses through the synergistic effects of the superficial chemistry and micro topography. To evaluate the respective in vitro cellular responses on the superficial chemistry and micro topography of titanium surface processed by wire-type electric discharge, we used titanium-coated epoxy resin replica of the surface. An oxide layer on the titanium surface processed by wire-type electric discharge activated the initial responses of osteoblastic cells through an integrin-mediated mechanism. Since the mRNA expression of ALP on those replicas was up-regulated compared to smooth titanium samples, the micro topography of a titanium surface processed by wire-type electric discharge promotes the osteogenic potential of cells. The synergistic response of the superficial chemistry and micro topography of titanium processed by wire-type electric discharge was demonstrated in this study.
线切割放电加工已应用于骨内钛植入物的制造，因为这种与计算机相关的技术能够在加工过程中实现极其精确的复杂样品成型，并具有最佳的微纹理表面。由于每次加工都会使氧化钛层发生敏感变化，作者推测该技术还通过表面化学和微观形貌的协同作用上调生物学反应。为了评估线切割放电加工的钛表面的表面化学和微观形貌各自的体外细胞反应，我们使用了该表面的钛涂层环氧树脂复制品。线切割放电加工的钛表面上的氧化层通过整合素介导的机制激活了成骨细胞的初始反应。由于与光滑钛样品相比，这些复制品上碱性磷酸酶（ALP）的mRNA表达上调，线切割放电加工的钛表面的微观形貌促进了细胞的成骨潜能。本研究证明了线切割放电加工的钛的表面化学和微观形貌的协同反应。
