Institute of Pharmacology and Toxicology, University of Zürich, Zürich, Switzerland.
J Cereb Blood Flow Metab. 2011 Aug;31(8):1668-74. doi: 10.1038/jcbfm.2011.84. Epub 2011 Jun 8.
Astrocytes have various important roles in brain physiology. To further elucidate the details of astrocytic functions under normal and pathological states, astrocyte-specific measurements are mandatory. For studying brain energy metabolism, the use of the astrocyte-specific energy substrate acetate has proven to be of great value. Since the first applications of labeled acetate for brain studies about 50 years ago, numerous methodologies have been developed and employed in compartment-specific investigations of brain metabolism. Here, we provide an overview of these different methodological approaches and review studies employing acetate labeled with the most commonly used carbon isotopes.
星形胶质细胞在脑生理学中具有多种重要作用。为了进一步阐明正常和病理状态下星形胶质细胞功能的细节，必须进行星形胶质细胞特异性测量。在研究脑能量代谢时，使用星形胶质细胞特异性能量底物醋酸盐已被证明具有重要价值。自大约 50 年前首次将标记的醋酸盐应用于脑研究以来，已经开发并采用了许多方法学方法，用于脑代谢的特定隔室研究。在这里，我们提供了这些不同方法学方法的概述，并回顾了使用最常用的碳同位素标记的醋酸盐进行的研究。
