Laboratorio de Endocrinología, Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina, Paraguay 2155, Piso 7, Buenos Aires, Argentina.
J Physiol Biochem. 2011 Dec;67(4):559-67. doi: 10.1007/s13105-011-0102-6. Epub 2011 Jun 9.
The aim of the present study was to investigate the effects of bisphenol A (BPA) on the neuroendocrine mechanism of control of the reproductive axis in adult male rats exposed to it during pre- and early postnatal periods. Wistar mated rats were treated with either 0.1% ethanol or BPA in their drinking water until their offspring were weaned at the age of 21 days. The estimated average dose of exposure to dams was approximately 2.5 mg/kg body weight per day of BPA. After 21 days, the pups were separated from the mother and sacrificed on 70 day of life. Gn-RH and gamma-aminobutyric acid (GABA) release from hypothalamic fragments was measured. LH, FSH, and testosterone concentrations were determined, and histological and morphometrical studies of testis were performed. Gn-RH release decreased significantly, while GABA serum levels were markedly increased by treatment. LH serum levels showed no changes, and FSH and testosterone levels decreased significantly. Histological studies showed abnormalities in the tubular organization of the germinal epithelium. The cytoarchitecture of germinal cells was apparently normal, and a reduction of the nuclear area of Leydig cells but not their number was observed. Taken all together, these results provide evidence of the effect caused by BPA on the adult male reproductive axis when exposed during pre- and postnatal period. Moreover, our findings suggest a probable GABA involvement in its effect at the hypothalamic level.
本研究旨在探讨双酚 A（BPA）对雄性幼鼠生殖轴神经内分泌调控机制的影响，这些幼鼠在出生前和出生后早期接触 BPA。Wistar 交配大鼠在饮用水中接受 0.1%乙醇或 BPA 处理，直到其后代在 21 天大时断奶。估计母体每天暴露于 BPA 的平均剂量约为 2.5mg/kg 体重。21 天后，幼鼠与母亲分离，并在 70 日龄时处死。测量下丘脑片段中 Gn-RH 和γ-氨基丁酸（GABA）的释放。测定 LH、FSH 和睾酮浓度，并对睾丸进行组织学和形态计量学研究。Gn-RH 释放显著减少，而 GABA 血清水平明显升高。LH 血清水平无变化，FSH 和睾酮水平显著降低。组织学研究显示生精上皮小管组织异常。生殖细胞的细胞结构显然正常，但观察到 Leydig 细胞的核面积减少而数量没有减少。综上所述，这些结果为 BPA 在出生前和出生后暴露于雄性生殖轴时产生的影响提供了证据。此外，我们的研究结果表明，GABA 可能参与了其在下丘脑水平的作用。
