Department of Gynecology, The Affiliated Tumor Hospital, Harbin Medical University, Harbin, China.
Cancer. 2011 Jun 15;117(12):2652-8. doi: 10.1002/cncr.25833. Epub 2011 Jan 10.
Lysosome-associated protein transmembrane 4 beta (LAPTM4B), a novel oncoprotein, is overexpressed in several carcinomas. Previous studies revealed that LAPTM4B polymorphisms contribute to the risk of certain types of cancers. The purpose of this study was to investigate the association between different LAPTM4B alleles and the risk of cervical carcinoma.
A case-control analysis was performed in 317 patients with cervical cancer and 416 control subjects. Genomic DNA was extracted from peripheral blood lymphocytes in all participants. LAPTM4B genotypes were determined using polymerase chain reaction. Odds ratios and 95% confidence intervals (CIs) were computed using an unconditional logistic regression model.
There was a significant difference (P<.001) in LAPTM4B2 allele frequency between cervical cancer cases (35.8%) and controls (26.3%). There was also a significant difference in the overall genotypic distribution between patients and controls (P < .001). Using the LAPTM4B1/1 genotype as a reference, we found that LAPTM4B allelic variation was associated with a significantly increased risk of cervical cancer, with adjusted odds ratios of 1.60 (95% CI, 1.15-2.22) and 2.12 (95% CI, 1.20-3.76) for the *1/2 and *2/2 genotype, respectively. Stratification analysis indicated that the association was more pronounced in younger subjects, smokers, premenopausal women, and women with more parities. Moreover, multiplicative joint effects were found between the *1/2 or *2/2 genotype and smoking.
The findings of this study indicated that the LAPTM4B*2 allele might be a cervical cancer risk factor and may play an important role in genetic susceptibility to cervical cancer in the Chinese population.
溶酶体相关蛋白跨膜 4β（LAPTM4B）是一种新型癌蛋白，在几种癌中过表达。先前的研究表明，LAPTM4B 多态性与某些类型癌症的风险有关。本研究旨在探讨不同 LAPTM4B 等位基因与宫颈癌风险之间的关系。
在 317 例宫颈癌患者和 416 例对照中进行病例对照分析。所有参与者的外周血淋巴细胞均提取基因组 DNA。采用聚合酶链反应检测 LAPTM4B 基因型。使用无条件逻辑回归模型计算比值比和 95%置信区间（CI）。
宫颈癌病例（35.8%）和对照组（26.3%）之间 LAPTM4B2 等位基因频率存在显著差异（P<.001）。患者和对照组之间的总体基因型分布也存在显著差异（P<.001）。以 LAPTM4B1/1 基因型为参考，我们发现 LAPTM4B 等位基因变异与宫颈癌风险显著增加相关，调整后的比值比分别为 1.60（95%CI，1.15-2.22）和 2.12（95%CI，1.20-3.76）。分层分析表明，这种关联在年轻受试者、吸烟者、绝经前妇女和生育次数较多的妇女中更为明显。此外，还发现了1/2 或2/2 基因型与吸烟之间的相乘联合效应。
本研究结果表明，LAPTM4B*2 等位基因可能是宫颈癌的危险因素，在中国人群中可能在宫颈癌遗传易感性中发挥重要作用。
