通过凝集素对人卵巢囊肿液中糖蛋白成分进行生化特性分析。

Biochemical characterization of glycoprotein components in human ovarian cyst fluids by lectins.

Yanagi K, Ohyama K, Yamakawa T, Watanabe H, Hirakawa S, Ohkuma S

Department of Biochemistry, Tokyo College of Pharmacy, Japan.

Int J Biochem. 1990;22(6):659-63. doi: 10.1016/0020-711x(90)90045-5.

Abstract

Perchloric acid-soluble glycoprotein fractions (PASFs) were separated, in yields of 180-610 mg per 100 ml of cyst fluid, from the cyst fluids of human ovarian cystadenomas (OCAs) in benign and borderline and ovarian clear cell carcinoma (OCC) in malignant, and then identified as glycoproteins with 19.1-69.5% carbohydrate by their chemical composition analyses. 2. These PASFs reacted with anti-A, -B, -Lea and/or -Leb sera, but did not react with anti-H, -M and -N sera. All of the PASFs reacted with Ricinus communis lectin (RCA-I). Among these PASFs, PASFs from the cyst fluids of OCA in benign did not react with Arachis hypogaea anti-T lectin (PNA) and both of the anti-N lectins of Vicia graminea (VGA) and Vicia unijuga (VUA), and PASFs from OCA in borderline reacted with only PNA and PASFs from OCC in malignant reacted with the three lectins, PNA, VGA and VUA. However, none of PASFs from human normal sera and various normal organ tissues reacted with any of RCA, PNA, VGA and VUA. 3. The above-mentioned results seem to show that the examination of glycoproteins of ovarian cyst fluids by the combined use of RCA-I, PNA and VGA (or VUA) permits biochemical diagnosis of the canceration degree of ovarian cystomas.

摘要

从良性和交界性人卵巢囊腺瘤（OCA）以及恶性卵巢透明细胞癌（OCC）的囊液中分离出高氯酸可溶性糖蛋白组分（PASF），每100毫升囊液的产量为180 - 610毫克，然后通过化学成分分析将其鉴定为碳水化合物含量为19.1% - 69.5%的糖蛋白。2. 这些PASF与抗A、抗B、抗Lea和/或抗Leb血清发生反应，但不与抗H、抗M和抗N血清发生反应。所有PASF都与蓖麻凝集素（RCA-I）发生反应。在这些PASF中，良性OCA囊液中的PASF不与花生凝集素（PNA）以及蚕豆（VGA）和窄叶野豌豆（VUA）的两种抗N凝集素发生反应，交界性OCA的PASF仅与PNA发生反应，恶性OCC的PASF与PNA、VGA和VUA这三种凝集素都发生反应。然而，来自人正常血清和各种正常器官组织的PASF均不与RCA、PNA、VGA和VUA中的任何一种发生反应。3. 上述结果似乎表明，联合使用RCA-I、PNA和VGA（或VUA）对卵巢囊液糖蛋白进行检测可对卵巢囊肿的癌变程度进行生化诊断。