Hagiwara T, Ohyama K, Yamakawa T, Watanabe H, Hirakawa S, Ohkuma S
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Yakugaku Zasshi. 1993 Nov;113(11):792-802. doi: 10.1248/yakushi1947.113.11_792.
Perchloric acid-soluble fractions (PASFs 1-3) which were obtained from cyst fluids of two patients [(Nos. 1 (blood group B) and 2 (blood group A))] with ovarian mucinous cystadenoma and one patient (No. 3 (blood group A)) with ovarian dermoid cyst in benign, were identified as glycoproteins by their chemical composition analyses, respectively. Reactivities of PASFs 1-3 against blood group anti-sera and lectins suggest that these PASFs did not contain MN blood group substances, Vgu glycoproteins and T-active glycoproteins. PASF 1 was separated into 11 fractions by Sephacryl S-500 gel filtration. From chemical composition, SDS-PAGE and serological property of the main fraction (Fr. 5), other one fraction (Fr. 7) and mild acid hydrolysates of these two fractions, it is possible to presume that PASF 1 consisted of a large number of AB-active and fucose-rich glycoproteins with high molecular weights and some of these glycoproteins bear either NeuNAc alpha 2-->6GalNAc (sialyl Tn) group or both sialyl Tn and sialylated T groups.
从两名患有卵巢黏液性囊腺瘤的患者[（1号患者（B血型）和2号患者（A血型））]以及一名患有良性卵巢皮样囊肿的患者（3号患者（A血型））的囊液中获得的高氯酸可溶性组分（PASF 1 - 3），通过化学成分分析分别鉴定为糖蛋白。PASF 1 - 3与血型抗血清和凝集素的反应表明，这些PASF不含有MN血型物质、Vgu糖蛋白和T活性糖蛋白。PASF 1通过Sephacryl S - 500凝胶过滤分离为11个组分。根据主要组分（第5组分）、另一个组分（第7组分）以及这两个组分的温和酸水解产物的化学成分、SDS - PAGE和血清学性质，可以推测PASF 1由大量具有高分子量的AB活性和富含岩藻糖的糖蛋白组成，其中一些糖蛋白带有NeuNAcα2→6GalNAc（唾液酸Tn）基团或同时带有唾液酸Tn和唾液酸化T基团。
