Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju 690-756, Korea.
Korean J Physiol Pharmacol. 2011 Apr;15(2):89-94. doi: 10.4196/kjpp.2011.15.2.89. Epub 2011 Apr 30.
Fucoidan is a sulfated polysaccharide derived from brown algae that has been reported to perform multiple biological activities, including immunostimulation. In this study, we investigated whether fucoidan has beneficial effects on endotoxemia induced by LPS, a septic model in mice. The focus of this study was on survival rates and spleen function of the mice upon treatment. We found that fucoidan had prophylactic effects on the survival rate of mice with endotoxemia. Flow cytometric analysis using antibodies for subset-specific markers revealed that fucoidan profoundly reversed the depleted population of dendritic cells in mice with endotoxemia. According to Western blot analysis, the spleen cells of LPS/fucoidan-treated mice showed a higher expression of anti-apoptotic molecules compared to those of LPS-treated mice. Also, fucoidan-treated spleen cells were more responsive to mitogens. Taken together, these results demonstrate that fucoidan pre-treatment has beneficial effects on the survival rate and function of the spleen in mice with endotoxemia. This study may broaden the use of fucoidan in clinical fields, especially endotoxemia.
岩藻聚糖是一种从褐藻中提取的硫酸多糖，已被报道具有多种生物活性，包括免疫刺激作用。在这项研究中，我们研究了岩藻聚糖是否对 LPS 诱导的内毒素血症（一种小鼠败血症模型）有有益作用。本研究的重点是治疗后小鼠的存活率和脾脏功能。我们发现岩藻聚糖对内毒素血症小鼠的存活率具有预防作用。使用针对亚群特异性标志物的抗体进行流式细胞术分析表明，岩藻聚糖可显著逆转内毒素血症小鼠中耗尽的树突状细胞群体。根据 Western blot 分析，LPS/岩藻聚糖处理的小鼠的脾细胞中抗凋亡分子的表达高于 LPS 处理的小鼠。此外，岩藻聚糖处理的脾细胞对有丝分裂原的反应更敏感。总之，这些结果表明岩藻聚糖预处理对内毒素血症小鼠的存活率和脾脏功能有有益作用。这项研究可能会拓宽岩藻聚糖在临床领域的应用，特别是在内毒素血症方面。
