Kim Su-Yeon, Joo Hong-Gu
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea.
J Vet Sci. 2015;16(2):145-50. doi: 10.4142/jvs.2015.16.2.145. Epub 2014 Dec 24.
Fucoidan is a sulfated polysaccharide derived from brown seaweed, including Fucus vesiculosus. This compound is known to have immunostimulatory effects on various types of immune cells including macrophages and dendritic cells. A recent study described the application of fucoidan as a vaccine adjuvant. Vaccination is regarded as the most efficient prophylactic method for preventing harmful or epidemic diseases. To increase vaccine efficacy, effective adjuvants are needed. In the present study, we determined whether fucoidan can function as an adjuvant using vaccine antigens. Flow cytometric analysis revealed that fucoidan increases the expression of the activation markers major histocompatibility complex class II, cluster of differentiation (CD)25, and CD69 in spleen cells. In combination with Bordetella bronchiseptica antigen, fucoidan increased the viability and tumor necrosis factor-α production of spleen cells. Furthermore, fucoidan increased the in vivo production of antigen-specific antibodies in mice inoculated with Mycoplasma hyopneumoniae antigen. Overall, this study has provided valuable information about the use of fucoidan as a vaccine adjuvant.
岩藻依聚糖是一种从褐藻（包括墨角藻）中提取的硫酸化多糖。已知该化合物对包括巨噬细胞和树突状细胞在内的各种免疫细胞具有免疫刺激作用。最近的一项研究描述了岩藻依聚糖作为疫苗佐剂的应用。接种疫苗被认为是预防有害或流行性疾病最有效的预防方法。为了提高疫苗效力，需要有效的佐剂。在本研究中，我们使用疫苗抗原确定岩藻依聚糖是否能作为佐剂发挥作用。流式细胞术分析显示，岩藻依聚糖可增加脾细胞中主要组织相容性复合体II类、分化簇（CD）25和CD69等活化标志物的表达。与支气管败血波氏杆菌抗原联合使用时，岩藻依聚糖可提高脾细胞的活力和肿瘤坏死因子-α的产生。此外，岩藻依聚糖可增加接种猪肺炎支原体抗原的小鼠体内抗原特异性抗体的产生。总体而言，本研究为岩藻依聚糖作为疫苗佐剂的应用提供了有价值的信息。
