从患冬季痢疾的奶牛中分离出的一种冠状病毒的细胞培养繁殖
Cell culture propagation of a coronavirus isolated from cows with winter dysentery.
作者信息
Benfield D A, Saif L J
机构信息
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
出版信息
J Clin Microbiol. 1990 Jun;28(6):1454-7. doi: 10.1128/jcm.28.6.1454-1457.1990.
Abstract
Fecal filtrates from cows with winter dysentery were inoculated into gnotobiotic and conventional calves, and a coronavirus was isolated from calf feces. Cytopathic effects were observed on human rectal tumor cells but not bovine cell cultures. The winter dysentery isolates morphologically and antigenically resembled the Mebus strain of bovine coronavirus.
摘要
将患有冬季痢疾的奶牛的粪便滤液接种到无菌和常规饲养的犊牛体内，从犊牛粪便中分离出一种冠状病毒。在人直肠肿瘤细胞上观察到细胞病变效应，但在牛细胞培养物上未观察到。冬季痢疾分离株在形态和抗原性上与牛冠状病毒的Mebus株相似。
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