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从患冬季痢疾的奶牛中分离出的一种冠状病毒的细胞培养繁殖

Cell culture propagation of a coronavirus isolated from cows with winter dysentery.

作者信息

Benfield D A, Saif L J

机构信息

Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.

出版信息

J Clin Microbiol. 1990 Jun;28(6):1454-7. doi: 10.1128/jcm.28.6.1454-1457.1990.

DOI:10.1128/jcm.28.6.1454-1457.1990
PMID:2166085
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC267955/
Abstract

Fecal filtrates from cows with winter dysentery were inoculated into gnotobiotic and conventional calves, and a coronavirus was isolated from calf feces. Cytopathic effects were observed on human rectal tumor cells but not bovine cell cultures. The winter dysentery isolates morphologically and antigenically resembled the Mebus strain of bovine coronavirus.

摘要

将患有冬季痢疾的奶牛的粪便滤液接种到无菌和常规饲养的犊牛体内，从犊牛粪便中分离出一种冠状病毒。在人直肠肿瘤细胞上观察到细胞病变效应，但在牛细胞培养物上未观察到。冬季痢疾分离株在形态和抗原性上与牛冠状病毒的Mebus株相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2506/267955/b6c33451eb73/jcm00054-0391-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2506/267955/1969fa7a0784/jcm00054-0390-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2506/267955/e3d01814ad73/jcm00054-0391-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2506/267955/b6c33451eb73/jcm00054-0391-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2506/267955/1969fa7a0784/jcm00054-0390-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2506/267955/e3d01814ad73/jcm00054-0391-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2506/267955/b6c33451eb73/jcm00054-0391-b.jpg

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本文引用的文献

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Bovine Virus Enteritis (Winter Dysentery).牛病毒性肠炎（冬季痢疾）
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Winter dysentery in dairy cattle.奶牛冬季痢疾
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Enhancement of plaque formation and cell fusion of an enteropathogenic coronavirus by trypsin treatment.胰蛋白酶处理增强肠道致病性冠状病毒的斑块形成和细胞融合。
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Trypsin-enhanced replication of neonatal calf diarrhea coronavirus in bovine embryonic lung cells.胰蛋白酶增强新生牛腹泻冠状病毒在牛胚胎肺细胞中的复制。
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