Benfield D A, Stotz I, Moore R, McAdaragh J P
J Clin Microbiol. 1982 Jul;16(1):186-90. doi: 10.1128/jcm.16.1.186-190.1982.
The purpose of this study was to determine whether sows shed rotavirus near the time of farrowing. Twelve sows purchased from a common source and seropositive for rotavirus were housed in isolation in farrowing crates from 5 days before to 3 weeks after farrowing. Fecal samples were collected at 3- to 4-day intervals and examined for the presence of rotavirus by direct electron microscopy. Samples were also treated with pancreatin and inoculated onto monkey kidney cells. Rotaviral antigens were detected by a direct immunofluorescence technique, and selected positive cultures were examined by immunoelectron microscopy. Rotavirus was detected in the feces of 5 of 12 sows as early as 5 days before to 2 weeks after farrowing. Diarrhea related to rotavirus developed in 4 of 12 litters. Two of these four litters were farrowed by sows which shed rotavirus at 7 and 10 and 14 days after farrowing. The results of this study indicate that sows immune to rotavirus can shed virus in their feces at a time when piglets are particularly susceptible to infection and that adult swine are of primary importance in the epidemiology of rotavirus as initiators of infection.
本研究的目的是确定母猪在分娩前后是否会排出轮状病毒。从同一来源购买的12头轮状病毒血清阳性母猪，在分娩前5天至分娩后3周单独饲养在分娩栏中。每隔3至4天采集粪便样本，通过直接电子显微镜检查是否存在轮状病毒。样本还用胰蛋白酶处理后接种到猴肾细胞上。通过直接免疫荧光技术检测轮状病毒抗原，对选定的阳性培养物进行免疫电子显微镜检查。在12头母猪中，有5头在分娩前5天至分娩后2周的粪便中检测到轮状病毒。12窝仔猪中有4窝出现了与轮状病毒相关的腹泻。这四窝中有两窝的母猪在分娩后7天、10天和14天排出了轮状病毒。本研究结果表明，对轮状病毒免疫的母猪在仔猪特别易受感染时可通过粪便排出病毒，成年猪作为感染的起始者在轮状病毒流行病学中至关重要。
