Lindop G B, Duncan K, Millan D W, Gibson A A, Patrick W J, Leckie B J, Birnie G D
University of Glasgow, Department of Pathology, Western Infirmary.
J Pathol. 1990 Jun;161(2):93-7. doi: 10.1002/path.1711610202.
Most cases of nephroblastoma have high plasma levels of prorenin which is biologically inactive. Plasma prorenin levels fall to normal following nephrectomy. In order to ascertain whether renin synthesis occurs in nephroblastomas we decided to search for renin-specific mRNA using a cDNA probe and Northern blot analyses on total RNA purified from snap-frozen human tumour tissue obtained at nephrectomy. We demonstrated renin-specific mRNA in 5/11 (45 per cent) nephroblastomas. It was 1.6 Kb in length, similar to the mRNA detected in normal kidney tissue and in kidneys with renal artery stenosis. In one of the cases of nephroblastoma, in which we could detect no normal renin mRNA at 1.6 Kb, the cDNA probe hybridized with a higher molecular weight mRNA 3 Kb in length. We conclude that some nephroblastomas synthesize renin.
大多数肾母细胞瘤患者的血浆中无生物活性的肾素原水平较高。肾切除术后血浆肾素原水平降至正常。为了确定肾母细胞瘤中是否发生肾素合成，我们决定使用cDNA探针并对从肾切除术中获取的速冻人肿瘤组织中纯化的总RNA进行Northern印迹分析，以寻找肾素特异性mRNA。我们在11例肾母细胞瘤中有5例（45%）检测到肾素特异性mRNA。其长度为1.6kb，与正常肾组织和肾动脉狭窄肾脏中检测到的mRNA相似。在其中一例肾母细胞瘤中，我们未检测到1.6kb的正常肾素mRNA，但cDNA探针与一个长度为3kb的分子量更高的mRNA杂交。我们得出结论，一些肾母细胞瘤可合成肾素。
