Rakin A V, Rykova V A
Mol Gen Mikrobiol Virusol. 1990 May(5):24-7.
The possibility to clone the structural leu gene of Yersinia pestis in vivo using the mini-Mu bacteriophages with the inserted plasmid replicones has been demonstrated. The E. coli K12 transductants having obtained the Leu(+)-marker within the cloned 4.8-21 kb fragments stably inherited the leu-gene within the autonomous mini-Mu replicones. The possibility to clone other Yersinia pestis genes by the same technique has been demonstrated.
已经证明了使用带有插入质粒复制子的微型Mu噬菌体在体内克隆鼠疫耶尔森菌结构亮氨酸基因的可能性。在克隆的4.8 - 21 kb片段中获得亮氨酸(+)标记的大肠杆菌K12转导子在自主微型Mu复制子内稳定地遗传了亮氨酸基因。已经证明了通过相同技术克隆其他鼠疫耶尔森菌基因的可能性。