Schrum D P, Culbertson C T, Jacobson S C, Ramsey J M
Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, Tennessee 37831-6142.
Anal Chem. 1999 Oct 1;71(19):4173-7. doi: 10.1021/ac990372u.
Flow cytometry of fluorescently labeled and unlabeled latex particles is demonstrated on a microfabricated device. The latex particles were detected and counted using laser light scattering and fluorescence coincidence measurements. Sample confinement was accomplished using electrokinetic focusing at a cross intersection, and detection occurred 50 μm downstream from the intersection. Particles with diameters of 1 and 2 μm were analyzed and distinguished from each other based on their light scattering intensity and fluorescence. A maximum sample throughput of 34 particles/s was achieved. Sample mixtures with varying proportions of fluorescently labeled and unlabeled particles were also analyzed and found to be within experimental error of the expected ratios.
在一个微制造设备上展示了对荧光标记和未标记乳胶颗粒的流式细胞术。使用激光光散射和荧光符合测量来检测和计数乳胶颗粒。通过在交叉路口处的电动聚焦实现样品限制，并在交叉路口下游50μm处进行检测。基于光散射强度和荧光对直径为1μm和2μm的颗粒进行分析并相互区分。实现了每秒34个颗粒的最大样品通量。还分析了具有不同比例荧光标记和未标记颗粒的样品混合物，发现其在预期比例的实验误差范围内。
