Wang J, Tian B, Sahlin E
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003.
Anal Chem. 1999 Sep 1;71(17):3901-4. doi: 10.1021/ac9904720.
An on-chip electrochemical detector for micromachined capillary electrophoresis (CE) systems, based on sputtering a gold working electrode directly onto the capillary outlet, is described. The new on-chip detector preparation requires no microfabrication or alignment procedures nor a decoupling mechanism. The attractive performance of the integrated electrophoresis chips/amperometric detection was demonstrated for the anodic detection of neurotransmitters. The response for dopamine was linear from 20 to 200 μM, with a LOD of 1.0 μM and a sensitivity of 52 pA/μM. Such intimate coupling of capillary electrophoresis chips and electrochemical detection facilitates the realization of complete integrated microanalytical devices.
本文描述了一种用于微机械毛细管电泳（CE）系统的片上电化学检测器，该检测器是通过将金工作电极直接溅射在毛细管出口上制成的。这种新型片上检测器的制备无需微加工、对准程序或去耦机制。集成电泳芯片/安培检测在神经递质的阳极检测中展现出了诱人的性能。多巴胺的响应在20至200μM范围内呈线性，检测限为1.0μM，灵敏度为52 pA/μM。毛细管电泳芯片与电化学检测的这种紧密耦合有助于实现完整的集成微分析装置。
