微芯片毛细管电泳的柱上电化学检测
On-column electrochemical detection for microchip capillary electrophoresis.
作者信息
Osbourn Damon M, Lunte Craig E
机构信息
Department of Chemistry, University of Kansas, 1251 Wescoe Hall Dr., Lawrence, Kansas 66045, USA.
出版信息
Anal Chem. 2003 Jun 1;75(11):2710-4. doi: 10.1021/ac026354l.
Abstract
The development of a cellulose acetate decoupler for on-column electrochemical detection in microchip capillary electrophoresis is presented. The capillary based laser-etched decoupler is translated to the planar format to isolate the detector circuit from the separation circuit. The decoupler is constructed by aligning a series of 20 30-microm holes through the coverplate of the microchip with the separation channel and casting a thin film of cellulose acetate within the holes. The decoupler shows excellent isolation of the detection circuit for separation currents up to 60 microA, with noise levels at or below 1 pA at a carbon fiber electrode. Detection limits of 25 nM were achieved for dopamine. This decoupler design combines excellent mechanical stability, effective shunting of high separation currents, and ease of manufacture.
摘要
本文介绍了一种用于微芯片毛细管电泳柱上电化学检测的醋酸纤维素去耦器的研制。基于毛细管的激光蚀刻去耦器被转换为平面形式，以将检测电路与分离电路隔离开来。通过将一系列20个30微米的孔穿过微芯片的盖板与分离通道对齐，并在孔内浇铸醋酸纤维素薄膜来构建去耦器。对于高达60微安的分离电流，该去耦器对检测电路显示出优异的隔离效果，在碳纤维电极处噪声水平为1皮安或更低。多巴胺的检测限达到25纳摩尔。这种去耦器设计兼具出色的机械稳定性、对高分离电流的有效分流以及易于制造的特点。
相似文献
1
On-column electrochemical detection for microchip capillary electrophoresis.微芯片毛细管电泳的柱上电化学检测
Anal Chem. 2003 Jun 1;75(11):2710-4. doi: 10.1021/ac026354l.
2
Cellulose acetate decoupler for on-column electrochemical detection in capillary electrophoresis.用于毛细管电泳柱上电化学检测的醋酸纤维素去偶联剂。
Anal Chem. 2001 Dec 15;73(24):5961-4. doi: 10.1021/ac010496i.
3
Development of a microfabricated palladium decoupler/electrochemical detector for microchip capillary electrophoresis using a hybrid glass/poly(dimethylsiloxane) device.使用玻璃/聚二甲基硅氧烷混合装置开发用于微芯片毛细管电泳的微型钯解耦器/电化学检测器。
Anal Chem. 2004 May 1;76(9):2482-91. doi: 10.1021/ac030327t.
4
Performance evaluation of a capillary electrophoresis electrochemical chip integrated with gold nanoelectrode ensemble working and decoupler electrodes.集成金纳米电极阵列工作电极和解耦电极的毛细管电泳电化学芯片的性能评估
J Chromatogr A. 2008 Jun 20;1194(2):231-6. doi: 10.1016/j.chroma.2008.04.056. Epub 2008 Apr 25.
5
A perfluorosulfonated ionomer end-column electrical decoupler for capillary electrophoresis/electrochemical detection.用于毛细管电泳/电化学检测的全氟磺酸离子交换末端柱电去耦器。
Anal Chem. 1995 Dec 1;67(23):4366-70. doi: 10.1021/ac00119a026.
6
On-column amperometric detection in capillary electrophoresis with an improved high-voltage electric field decoupler.采用改进型高压电场去耦器的毛细管电泳柱上安培检测法。
J Chromatogr A. 2000 Mar 3;872(1-2):259-68. doi: 10.1016/s0021-9673(99)01260-1.
7
Integration of a carbon microelectrode with a microfabricated palladium decoupler for use in microchip capillary electrophoresis/electrochemistry.用于微芯片毛细管电泳/电化学的碳微电极与微加工钯解耦器的集成。
Electrophoresis. 2005 Jan;26(1):202-10. doi: 10.1002/elps.200406188.
8
An integrated decoupler for capillary electrophoresis with electrochemical detection: application to analysis of brain microdialysate.一种用于毛细管电泳与电化学检测的集成去耦器：应用于脑微透析液分析。
Anal Chem. 1999 Oct 15;71(20):4486-92. doi: 10.1021/ac990338f.
9
Three-electrode electrochemical detector and platinum film decoupler integrated with a capillary electrophoresis microchip for amperometric detection.三电极电化学检测器和铂膜去耦器与毛细管电泳微芯片集成用于安培检测。
Anal Chem. 2003 Feb 15;75(4):947-52. doi: 10.1021/ac025912t.
10
Carbon paste-based electrochemical detectors for microchip capillary electrophoresis/electrochemistry.用于微芯片毛细管电泳/电化学的碳糊基电化学检测器。
Analyst. 2001 Mar;126(3):277-80. doi: 10.1039/b009827m.
引用本文的文献
1
Use of 3D printing to integrate microchip electrophoresis with amperometric detection.利用3D打印技术将微芯片电泳与安培检测相结合。
Anal Bioanal Chem. 2024 Sep;416(21):4749-4758. doi: 10.1007/s00216-024-05260-6. Epub 2024 Apr 6.
2
Biological applications of microchip electrophoresis with amperometric detection: in vivo monitoring and cell analysis.微芯片电泳与安培检测的生物应用：体内监测和细胞分析。
Anal Bioanal Chem. 2020 Sep;412(24):6101-6119. doi: 10.1007/s00216-020-02647-z. Epub 2020 Apr 28.
3
Fast and versatile fabrication of PMMA microchip electrophoretic devices by laser engraving.通过激光雕刻快速且多功能地制造聚甲基丙烯酸甲酯微芯片电泳装置
Electrophoresis. 2014 Aug;35(16):2325-32. doi: 10.1002/elps.201470140.
4
In-channel amperometric detection for microchip electrophoresis using a wireless isolated potentiostat.采用无线隔离式电化学工作站的微流控芯片电泳通道内安培检测
Electrophoresis. 2011 Apr;32(8):832-7. doi: 10.1002/elps.201000681. Epub 2011 Mar 16.
5
Amperometric detection in microchip electrophoresis devices: effect of electrode material and alignment on analytical performance.微芯片电泳设备中的安培检测：电极材料和对准度对分析性能的影响。
Electrophoresis. 2009 Oct;30(19):3324-33. doi: 10.1002/elps.200900317.
本文引用的文献
1
Integrated electrophoresis chips/amperometric detection with sputtered gold working electrodes.集成电泳芯片/采用溅射金工作电极的安培检测法
Anal Chem. 1999 Sep 1;71(17):3901-4. doi: 10.1021/ac9904720.
2
Micromachined electrophoresis chips with thick-film electrochemical detectors.带有厚膜电化学检测器的微机械电泳芯片。
Anal Chem. 1999 Dec 1;71(23):5436-40. doi: 10.1021/ac990807d.
3
Electrochemical detection for microscale analytical systems: a review.用于微尺度分析系统的电化学检测：综述
Talanta. 2002 Feb 11;56(2):223-31. doi: 10.1016/s0039-9140(01)00592-6.
4
Microchip capillary electrophoresis coupled with a boron-doped diamond electrode-based electrochemical detector.微芯片毛细管电泳与基于硼掺杂金刚石电极的电化学检测器联用。
Anal Chem. 2003 Feb 15;75(4):935-9. doi: 10.1021/ac0262053.
5
Microchip capillary electrophoresis with electrochemical detection.带有电化学检测的微芯片毛细管电泳
Anal Chem. 2002 May 15;74(10):2441-5. doi: 10.1021/ac0110247.
6
In-channel electrochemical detection for microchip capillary electrophoresis using an electrically isolated potentiostat.使用电隔离恒电位仪的微芯片毛细管电泳通道内电化学检测。
Anal Chem. 2002 Mar 1;74(5):1136-43. doi: 10.1021/ac011087p.
7
Cellulose acetate decoupler for on-column electrochemical detection in capillary electrophoresis.用于毛细管电泳柱上电化学检测的醋酸纤维素去偶联剂。
Anal Chem. 2001 Dec 15;73(24):5961-4. doi: 10.1021/ac010496i.
8
Microchip capillary electrophoresis/electrochemistry.微芯片毛细管电泳/电化学
Electrophoresis. 2001 Aug;22(12):2526-36. doi: 10.1002/1522-2683(200107)22:12<2526::AID-ELPS2526>3.0.CO;2-K.
9
Fabrication and evaluation of a carbon-based dual-electrode detector for poly(dimethylsiloxane) electrophoresis chips.用于聚二甲基硅氧烷电泳芯片的碳基双电极探测器的制备与评估
Electrophoresis. 2001 Jan;22(2):242-8. doi: 10.1002/1522-2683(200101)22:2<242::AID-ELPS242>3.0.CO;2-W.
10
Carbon paste-based electrochemical detectors for microchip capillary electrophoresis/electrochemistry.用于微芯片毛细管电泳/电化学的碳糊基电化学检测器。
Analyst. 2001 Mar;126(3):277-80. doi: 10.1039/b009827m.
Zotero 插件