Department of Chemistry and UF Brain Institute, University of Florida, Gainesville, Florida 32611.
Anal Chem. 1999 Aug 1;71(15):3101-5. doi: 10.1021/ac990225w.
We have developed a new fluorescent method for single-molecule detection (SMD) and imaging using an optical fiber probe. The fluorophores were excited by the evanescent wave field produced on the core surface of the optical fiber. This was achieved by exposing a section of the core of the optical fiber probe to the fluorophore solution. Both cylindrical and square optical fiber probes were used for SMD. The fluorescent signals were detected by an intensified charge-coupled device. Single rhodamine 6G molecules have been detected. The number of rhodamine 6G molecules imaged by the optical fiber probe showed an excellent linear relationship with the concentrations of the fluorophores. The SMD scheme was also applied to the imaging of biomolecules, such as molecular beacon DNA molecules, labeled with tetramethylrhodamine. Our results have shown that using an optical fiber is an easy yet effective approach to SMD. It represents a simpler fluorescent method for the detection of single-molecules in solution and at an interface.
我们开发了一种新的荧光单分子检测（SMD）和成像方法，使用光纤探针。荧光团通过光纤芯表面产生的消逝波场激发。这是通过将光纤探针的芯的一部分暴露于荧光团溶液来实现的。圆柱形和方形光纤探针都用于 SMD。荧光信号由增强型电荷耦合器件检测。已经检测到单个若丹明 6G 分子。光纤探针成像的若丹明 6G 分子数量与荧光团浓度呈极好的线性关系。SMD 方案还应用于生物分子的成像，例如用四甲基罗丹明标记的分子信标 DNA 分子。我们的结果表明，使用光纤是一种简单而有效的 SMD 方法。它代表了一种更简单的荧光方法，用于检测溶液中和界面处的单个分子。
