Shi Hui, He XiaoXiao, Yang XiaoHai, Wang KeMin, Wang Qing, Guo QiuPing, Huo XiQin
1State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082 China.
2College of Chemistry and Chemical Engineering, Biomedical Engineering Center, Hunan University, Changsha, 410082 China.
Sci China Chem. 2010;53(4):704-719. doi: 10.1007/s11426-010-0110-3. Epub 2010 May 20.
With the completion of the human genome-sequencing project, there has been a resulting change in the focus of studies from genomics to proteomics. By utilizing the inherent advantages of molecular beacon probes and biofunctionalized nanoparticles, a series of novel principles, methods and techniques have been exploited for bioanalytical and biomedical studies. This review mainly discusses the applications of molecular beacon probes and biofunctionalized nanoparticles-based technologies for real-time, , highly sensitive and highly selective protein analysis, including the nonspecific or specific protein detection and separation, protein/DNA interaction studies, cell surface protein recognition, and antigen-antibody binding process-based bacteria assays. The introduction of molecular beacon probes and biofunctionalized nanoparticles into the protein analysis area would necessarily advance the proteomics research.
随着人类基因组测序项目的完成，研究重点已从基因组学转向蛋白质组学。通过利用分子信标探针和生物功能化纳米颗粒的固有优势，已开发出一系列用于生物分析和生物医学研究的新原理、方法和技术。本综述主要讨论基于分子信标探针和生物功能化纳米颗粒的技术在实时、高灵敏度和高选择性蛋白质分析中的应用，包括非特异性或特异性蛋白质检测与分离、蛋白质/DNA相互作用研究、细胞表面蛋白质识别以及基于抗原-抗体结合过程的细菌检测。将分子信标探针和生物功能化纳米颗粒引入蛋白质分析领域必将推动蛋白质组学研究的发展。
