School of Biological Science, Washington State University, Pullman, Washington 99164-4236 USA;
Am J Bot. 2002 Apr;89(4):578-86. doi: 10.3732/ajb.89.4.578.
Application of 100 mmol/L methyl jasmonate (MJ) to the intact bark of 30-yr-old Norway spruce induced anatomical reactions related to defense. Within 30 d, a single MJ treatment induced swelling of existing polyphenolic parenchyma cells (PP cells) and an increase in their phenolic contents and formation of additional PP cells and of traumatic resin ducts (TDs) at the cambial zone. These changes occurred up to 7 cm away from the application zone. Treatment enhanced resin flow and increased resistance to the blue-stain fungus, Ceratocystis polonica. Methyl jasmonate application to the oldest internode of 2-yr-old saplings also induced TD formation, and, more surprisingly, TDs were formed in the untreated internode. Traumatic ducts were not formed in branches, ruling out an effect of volatile MJ on the upper internode. Methyl jasmonate application never gave rise to a hypersensitive response, cell death, tissue necrosis, or wound periderm, indicating the amount of MJ transported across the periderm was very low relative to the application concentration. This is the first report of a single compound giving rise to major cellular features related to acquired resistance and previously shown to be induced by wounding, fungal infection, and bark beetles in Norway spruce.
100mmol/L 茉莉酸甲酯（MJ）应用于 30 年生挪威云杉完整树皮会引起与防御相关的解剖学反应。在 30 天内，单次 MJ 处理会导致现有的多酚质薄壁细胞（PP 细胞）膨胀，其酚类物质含量增加，并在形成层区形成额外的 PP 细胞和创伤树脂导管（TD）。这些变化发生在距施药区 7cm 以内。处理增强了树脂流动并增加了对蓝变真菌 Ceratocystis polonica 的抗性。将 MJ 应用于 2 年生幼树的最老节间也会诱导 TD 形成，更令人惊讶的是，未处理的节间也形成了 TD。TD 未在树枝上形成，排除了挥发性 MJ 对上部节间的影响。MJ 处理从未引起过敏反应、细胞死亡、组织坏死或创伤周皮，表明穿过周皮运输的 MJ 量相对于施药浓度非常低。这是首次报道单一化合物会引起与获得性抗性相关的主要细胞特征，先前的研究表明，这些特征会因挪威云杉的创伤、真菌感染和树皮甲虫而被诱导。
