College of Animal Science and Technology, Nanjing Agricultural University, No 1 Weigang, Nanjing, 210095 Jiangsu, People's Republic of China.
Mol Biol Rep. 2012 Mar;39(3):2439-45. doi: 10.1007/s11033-011-0994-9. Epub 2011 Jun 12.
To examine the effect of Clusterin (CLU) on mastitis, genetic association analysis was applied on mastitis and milk production traits of 1,137 Chinese Holstein cows. We detected two novel single nucleotide polymorphisms (SNPs), G+15781A in the seventh exon and C-994T before 5'-upstream region (UTR) of CLU gene, found five TATA box, one CpG island and more transcription factor binding sites in promoter region, respectively, Milk fat rate in genotype AA was significantly higher than in GG on fat rate (P < 0.01), milk fat and milk yield in combined haplotype H1H4 (AGCT) were significantly higher than in H3H4 (GGCT) (P < 0.05), H1H4 was decided advantage in nine milk production traits. Quadruplet cows in G+15781A were decided advantage in each milk production traits that 305-day milk yield, fat rate, protein rate and somatic cell scores (SCS) increased following with birth order. A allele and T allele had positive effect on SCS. In conclusion, this study showed that the haplotype AA may be a genetic marker on mastitis and other performance for Chinese Holstein cows.
为了研究CLU 对乳腺炎的影响，我们对 1137 头中国荷斯坦奶牛的乳腺炎和产奶性状进行了遗传关联分析。我们在 CLU 基因的第七外显子和 5'-UTR 前发现了两个新的单核苷酸多态性（SNPs），G+15781A 和 C-994T，分别在启动子区域发现了五个 TATA 盒、一个 CpG 岛和更多的转录因子结合位点。AA 基因型的牛奶脂肪率显著高于 GG 基因型的脂肪率（P<0.01），AGCT 组合单倍型 H1H4 的牛奶脂肪和产奶量显著高于 GGCT 单倍型 H3H4（P<0.05），H1H4 在九个产奶性状中具有优势。在 G+15781A 中，四重奶牛在每个产奶性状中都具有优势，随着产仔顺序的增加，305 天产奶量、脂肪率、蛋白率和体细胞评分（SCS）增加。A 等位基因和 T 等位基因对 SCS 有正向影响。综上所述，本研究表明，AA 单倍型可能是中国荷斯坦奶牛乳腺炎和其他性能的遗传标记。
