Corn and Soybean Research Unit, USDA, Agricultural Research Service, Wooster, OH 44691, USA.
Theor Appl Genet. 2011 Sep;123(5):729-40. doi: 10.1007/s00122-011-1622-8. Epub 2011 Jun 12.
Genes on chromosomes six (Wsm1), three (Wsm2) and ten (Wsm3) in the maize (Zea mays L.) inbred line Pa405 control resistance to Wheat streak mosaic virus (WSMV), and the same or closely linked genes control resistance to Maize dwarf mosaic virus (MDMV) and Sugarcane mosaic virus (SCMV). Near isogenic lines (NIL) carrying one or two of the genes were developed by introgressing regions of the respective chromosomes into the susceptible line Oh28 and tested for their responses to WSMV, MDMV, and SCMV in the field and greenhouse. F(1) progeny from NIL × Oh28 were also tested. Wsm1, or closely linked genes, provided resistance to all three viruses, as determined by symptom incidence and severity. Wsm2 and Wsm3 provided resistance to WSMV. Wsm2 and/or Wsm3 provided no resistance to MDMV, but significantly increased resistance in plants with one Wsm1 allele. NIL carrying Wsm1, Wsm2, or Wsm3 had similar SCMV resistance in the field, but NIL with Wsm2 and Wsm3 were not resistant in the greenhouse. Addition of Wsm2 to Wsm1 increased SCMV resistance in the field. For all viruses, symptom incidence was higher in the greenhouse than in the field, and relative disease severity was higher in the greenhouse for WSMV and MDMV. An Italian MDMV isolate and the Ohio SCMV infected the Wsm1 NIL, while the Ohio MDMV and Seehausen SCMV isolates did not. Our results indicate that the three genes, or closely linked loci, provide virus resistance. Resistance conferred by the three genes is influenced by interactions among the genes, the virus species, the virus isolate, and the environment.
染色体六（Wsm1）、三（Wsm2）和十（Wsm3）上的基因控制玉米自交系 Pa405 对小麦条纹花叶病毒（WSMV）的抗性，相同或紧密连锁的基因控制对玉米矮花叶病毒（MDMV）和甘蔗花叶病毒（SCMV）的抗性。通过将相应染色体的区域导入感病系 Oh28，近等基因系（NIL）携带一个或两个基因得到了发展，并在田间和温室中测试了它们对 WSMV、MDMV 和 SCMV 的反应。还测试了 NIL×Oh28 的 F1 后代。Wsm1 或紧密连锁的基因，如症状发生率和严重程度所确定，对所有三种病毒均提供抗性。Wsm2 和 Wsm3 提供对 WSMV 的抗性。Wsm2 和/或 Wsm3 对 MDMV 没有抗性，但在携带一个 Wsm1 等位基因的植物中显著增加了抗性。携带 Wsm1、Wsm2 或 Wsm3 的 NIL 在田间具有相似的 SCMV 抗性，但在温室中 NIL 与 Wsm2 和 Wsm3 均不具有抗性。在温室中，Wsm2 的添加增加了 WSMV 的抗性。对于所有病毒，温室中的症状发生率高于田间，温室中 WSMV 和 MDMV 的相对严重程度较高。意大利 MDMV 分离物和俄亥俄 SCMV 感染了 Wsm1 NIL，而俄亥俄 MDMV 和 Seehausen SCMV 分离物则没有。我们的结果表明，这三个基因或紧密连锁的基因座提供了病毒抗性。三个基因赋予的抗性受到基因、病毒物种、病毒分离物和环境之间相互作用的影响。
