Jones M W, Redinbaugh M G, Louie R
United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Corn and Soybean Research, Wooster, OH 44691.
USDA-ARS, Corn and Soybean Research, Department of Plant Pathology, The Ohio State University, Wooster 44691.
Plant Dis. 2007 Feb;91(2):185-190. doi: 10.1094/PDIS-91-2-0185.
Previously, Mdm1, a gene controlling resistance to Maize dwarf mosaic virus (MDMV), was identified in the inbred line Pa405. The gene was tightly linked to the restriction fragment length polymorphism marker umc85 on the short arm of chromosome 6. This chromosomal region is also the location of resistance genes to two other viruses in the family Potyviridae, Sugarcane mosaic virus (SCMV) and Wheat streak mosaic virus (WSMV). A diverse collection of 115 maize inbred lines was evaluated for resistance to MDMV and SCMV, and for MDMV resistance loci on chromosome 6S. Forty-six resistant inbred lines were crossed to three MDMVsusceptible inbred lines to develop F populations. The F populations were inoculated with MDMV and scored for infection and symptom type. Environmental factors influenced both the rate and type of symptom development. Bulked segregant analysis of each F population indicated that, in 42 of 43 MDMV-resistant lines, chromosome 6S markers found in the resistant parent also were present in the bulked resistant but not the susceptible tissue. Markers previously associated with resistance to both SCMV and WSMV on chromosome 3 and to WSMV on chromosome 10 were associated with resistance in nine and seven of the F populations, respectively. These data suggest that Mdm1 or closely linked genes on chromosome 6S are associated with MDMV resistance in most germplasm, but that other loci also may affect resistance.
此前，在自交系Pa405中鉴定出一个控制玉米矮花叶病毒（MDMV）抗性的基因Mdm1。该基因与位于第6号染色体短臂上的限制性片段长度多态性标记umc85紧密连锁。这个染色体区域也是马铃薯Y病毒科另外两种病毒——甘蔗花叶病毒（SCMV）和小麦线条花叶病毒（WSMV）抗性基因的所在位置。对115个不同的玉米自交系进行了MDMV和SCMV抗性以及第6号染色体短臂上MDMV抗性位点的评估。将46个抗性自交系与3个感MDMV的自交系杂交，构建F群体。对F群体接种MDMV，并对感染情况和症状类型进行评分。环境因素影响症状发展的速度和类型。对每个F群体进行混合分离分析表明，在43个MDMV抗性品系中的42个中，抗性亲本中发现的第6号染色体短臂标记也存在于混合的抗性组织而非感病组织中。先前与第3号染色体上SCMV和WSMV抗性以及第10号染色体上WSMV抗性相关的标记，分别在9个和7个F群体中与抗性相关。这些数据表明，第6号染色体短臂上的Mdm1或紧密连锁的基因在大多数种质中与MDMV抗性相关，但其他位点也可能影响抗性。
