Biogenesi, Reproductive Medicine Center, Istituti Clinici Zucchi, Monza, Italy.
Fertil Steril. 2011 Aug;96(2):321-3. doi: 10.1016/j.fertnstert.2011.05.054. Epub 2011 Jun 12.
To assess the applicability of oocyte IVM in case of nonobstructive azoospermia (NOA).
Case series.
Private IVF unit.
PATIENT(S): All male partners were affected by NOA. Twenty-one women (20 normo-ovulatory and 1 with polycystic ovary) underwent 27 IVM treatments. The outcome of controlled ovarian stimulation (COS) cycles in normo-ovulatory women whose partners were affected by NOA was assessed for comparison.
INTERVENTION(S): Spermatozoa from testicular sperm extraction (TESE) samples were retrieved and cryopreserved before treatment.
MAIN OUTCOME MEASURE(S): Rates of fertilization, pregnancy per cycle and transfer, and implantation.
RESULT(S): Twenty-seven IVM-TESE cycles were carried out in 21 women. Fertilization rate was 64.4%. In compliance with national legislation, no more than three oocytes were inseminated in each cycle and all developing embryos were transferred. Six single clinical pregnancies were ascertained (22.2% and 27.3% pregnancy rates per cycle and per transfer, respectively). One pregnancy ended in abortion. The others developed to term and gave rise to five healthy babies. Sixty couples underwent standard COS-TESE treatment. Rates of fertilization, clinical pregnancy per cycle, implantation, and abortion were 64.6%, 20.0%, 11.3%, and 16.7%, respectively. Eleven babies were born from ten pregnancies.
CONCLUSION(S): Oocyte IVM may represent an option in NOA cases.
评估卵母细胞体外成熟（IVM）在非梗阻性无精子症（NOA）中的应用。
病例系列。
私人体外受精单位。
所有男性均患有 NOA。21 名女性（20 名正常排卵和 1 名多囊卵巢）接受了 27 次 IVM 治疗。对其配偶患有 NOA 的正常排卵女性的控制性卵巢刺激（COS）周期的结果进行了评估，以供比较。
TESE 样本中的精子被取出并冷冻保存，然后再进行治疗。
受精率、每个周期和移植的妊娠率以及胚胎着床率。
21 名女性共进行了 27 次 IVM-TESE 周期。受精率为 64.4%。根据国家法规，每个周期最多只能对 3 个卵子进行授精，并且所有发育中的胚胎都被移植。确定了 6 例单胎临床妊娠（分别为 22.2%和 27.3%的周期和移植妊娠率）。1 例妊娠流产。其余的妊娠均足月，并产生了 5 个健康婴儿。60 对夫妇接受了标准的 COS-TESE 治疗。受精率、每个周期的临床妊娠率、胚胎着床率和流产率分别为 64.6%、20.0%、11.3%和 16.7%。11 名婴儿分别来自 10 例妊娠。
卵母细胞 IVM 可能是 NOA 病例的一种选择。
