Matsuoka T, Naito T, Kubota Y, Morita Y, Takei J, Akiyama K, Hata J, Hasegawa A, Sata T, Kurata T
Department of Neonatology, National Children's Hospital, Tokyo, Japan.
Acta Paediatr Scand. 1990 May;79(5):568-71. doi: 10.1111/j.1651-2227.1990.tb11515.x.
A case of fatal disseminated adenovirus infection in a neonate who suffered from severe keratoconjunctivitis and pneumonitis is reported. The diagnosis was made seven days after the onset of illness based on the detection of adenovirus antigen in the smears of the tracheal suction and conjunctival swab by immunofluorescence. Viral antigen was detected in the frozen or formalin-fixed autopsy specimens of the lungs, kidneys, spleen, liver and lymph nodes. Typical crystal arrangement of adenovirus virions was observed in the alveolar epithelial cells by electron microscopy. The isolated virus was identified to be of type 19 by a neutralization test. The IF examination using adenovirus group specific immune reagents on the smears of clinical specimens appears to be useful for rapid diagnosis of viral infections.
报告了1例患有严重角结膜炎和肺炎的新生儿致命性播散性腺病毒感染病例。发病7天后，通过免疫荧光法在气管吸引物涂片和结膜拭子中检测到腺病毒抗原，从而做出诊断。在肺、肾、脾、肝和淋巴结的冷冻或福尔马林固定尸检标本中检测到病毒抗原。通过电子显微镜在肺泡上皮细胞中观察到腺病毒颗粒的典型晶体排列。通过中和试验鉴定分离出的病毒为19型。使用腺病毒组特异性免疫试剂对临床标本涂片进行免疫荧光检查似乎有助于病毒感染的快速诊断。
