Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):971-976. doi: 10.1099/ijs.0.033043-0. Epub 2011 Jun 13.
A Gram-positive-staining, non-motile, rod- or coccoid-shaped actinobacterium, designated strain H17T, was isolated from a soil sample from Nanjing, Jiangsu Province, PR China. The organism grew optimally at 30 °C, pH 7.0 and with 3 % NaCl (w/v). Strain H17T contained L-Lys-D-Asp as the cell-wall peptidoglycan type and galactose, xylose and mannose as the whole-cell sugars. The major fatty acids were anteiso-C15:0 and iso-C15:0. The total polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipids, phosphatidylinositol, phosphoglycolipid, one unidentified glycolipid and one unidentified lipid. The menaquinone was MK9(H4). Mycolic acids were not detected. The DNA G+C content was 72.4 mol%. Phylogenetic analysis of this strain based on 16S rRNA gene sequences revealed 97.8-99.6 % similarity to recognized species of the genus Isoptericola. The low level of DNA-DNA relatedness to other species of the genus Isoptericola and the many phenotypic properties that distinguished strain H17T from recognized species of this genus demonstrated that isolate H17T should be classified as representing a novel species of the genus Isoptericola. The name Isoptericola nanjingensis sp. nov. is proposed for this novel species. The type strain is H17T (=DSM 24300T=CCTCC AB 2011005T).
一株革兰氏阳性、不运动、杆状或球状放线菌，命名为 H17T 菌株，从中国江苏省南京市的土壤样本中分离得到。该菌在 30°C、pH7.0 和 3%NaCl（w/v）条件下生长最佳。H17T 菌株的细胞壁肽聚糖类型为 L-Lys-D-Asp，全细胞糖为半乳糖、木糖和甘露糖。主要脂肪酸为 anteiso-C15:0 和 iso-C15:0。总极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂、磷脂酰肌醇、磷糖脂、一种未鉴定的糖脂和一种未鉴定的脂类。主要醌为 MK9(H4)。未检测到分枝菌酸。DNA G+C 含量为 72.4mol%。基于 16S rRNA 基因序列的系统发育分析表明，该菌株与公认的异孢菌属物种的相似度为 97.8-99.6%。与异孢菌属其他物种的 DNA-DNA 同源性较低，以及与该属其他已知种在许多表型特性上的差异，表明 H17T 菌株应归类为代表异孢菌属的一个新种。提议将该新种命名为南京异孢菌（Isoptericola nanjingensis）sp. nov.。其模式菌株为 H17T（=DSM 24300T=CCTCC AB 2011005T）。
