College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
The Key Laboratory for Microbial Resources of Ministry of Education, and Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Int J Syst Evol Microbiol. 2010 Apr;60(Pt 4):904-908. doi: 10.1099/ijs.0.012864-0. Epub 2009 Aug 6.
A Gram-positive-staining, non-motile, rod- or coccoid-shaped bacterium, strain CLG(T), was isolated from beach sand from Lianyungang, Jiangsu Province, PR China. The organism grew optimally at 30 degrees C and pH 7.0. Strain CLG(T) contained L-Lys-D-Asp as the peptidoglycan type and galactose, rhamnose and xylose as the whole-cell sugars. It contained anteiso-C(15 : 0) and iso-C(15 : 0) as the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, phosphatidylinositol, three phosphoglycolipids and one unidentified lipid. The DNA G+C content was 70.3 mol%. The 16S rRNA gene sequence similarity of strain CLG(T) with Isoptericola dokdonensis DS-3(T) was 99.4 % but the DNA-DNA relatedness between strain CLG( T) and I. dokdonensis DS-3(T) was 58.7 %. There were also many phenotypic properties that distinguished strain CLG( T) from recognized species of the genus Isoptericola. The results showed that strain CLG(T) represents a novel species of the genus Isoptericola, for which the name Isoptericola jiangsuensis sp. nov. is proposed. The type strain is CLG(T) (=DSM 21863( T)=CCTCC AB208287(T)).
一株革兰氏阳性、不运动、杆状或球状的细菌，菌株 CLG(T)，从中国江苏省连云港的海滩沙中分离得到。该生物在 30°C 和 pH 7.0 时生长最佳。菌株 CLG(T)含有 L-Lys-D-Asp 作为肽聚糖类型，以及半乳糖、鼠李糖和木糖作为全细胞糖。它含有 anteiso-C(15:0)和 iso-C(15:0)作为主要脂肪酸。主要极性脂类是双磷脂酰甘油、磷脂酰甘油、磷脂、磷脂酰肌醇、三种磷酸甘油酯和一种未鉴定的脂类。DNA G+C 含量为 70.3 mol%。菌株 CLG(T)与 Isoptericola dokdonensis DS-3(T)的 16S rRNA 基因序列相似度为 99.4%，但菌株 CLG(T)与 I.dokdonensis DS-3(T)之间的 DNA-DNA 相关性为 58.7%。菌株 CLG(T)与已鉴定的鞘翅目属的许多表型特性也有区别。结果表明，菌株 CLG(T)代表鞘翅目属的一个新种，建议将其命名为鞘翅目属 Jiangsuensis sp. nov.。其模式菌株为 CLG(T) (=DSM 21863(T)=CCTCC AB208287(T))。
