Perino L J, Wright R E, Hoppe K L, Fulton R W
Department of Veterinary Parasitology, Oklahoma State University, Stillwater 74078.
Am J Vet Res. 1990 Aug;51(8):1167-9.
A successful attempt was made to mechanically transmit bovine leukosis virus (BLV) from a BLV-infected cow with a normal lymphocyte count to sheep by inoculation with horse fly (Tabanus abactor) mouthparts. After interrupted natural feeding, horse flies were anesthetized with CO2. Mouthparts were severed and pooled into a tissue grinder containing medium. Five inocula containing the mouthparts of 10 flies each, and 5 inocula containing the mouthparts of 20 flies each, were prepared and inoculated SC in the right axilla of 10 BLV antibody-negative sheep. Five additional sheep served as controls. Serum samples were collected at 2-week intervals and tested by agar gel immunodiffusion for BLV antibodies. One sheep injected with 20 mouthparts developed antibodies to BLV at 10 weeks after inoculation. Six months after inoculation with fly mouthparts, 1 BLV antibody-negative sheep was randomly selected from each treatment group and injected, in the left axilla, with 3 ml of blood from the donor cow to confirm susceptibility of the sheep. All 3 sheep developed antibodies to BLV within 4 weeks.
通过用厩螫蝇（Tabanus abactor）口器接种，成功尝试将牛白血病病毒（BLV）从一只淋巴细胞计数正常的BLV感染牛机械传播给绵羊。在自然取食中断后，用二氧化碳麻醉厩螫蝇。将口器切断并汇集到含有培养基的组织研磨器中。制备了5个接种物，每个接种物含有10只苍蝇的口器，以及5个接种物，每个接种物含有20只苍蝇的口器，并将它们皮下接种到10只BLV抗体阴性绵羊的右腋窝。另外5只绵羊作为对照。每隔2周采集血清样本，并通过琼脂凝胶免疫扩散法检测BLV抗体。一只注射了20个口器的绵羊在接种后10周产生了BLV抗体。在用苍蝇口器接种6个月后，从每个治疗组中随机选择1只BLV抗体阴性绵羊，在其左腋窝注射3毫升供体牛的血液，以确认绵羊的易感性。所有3只绵羊在4周内都产生了BLV抗体。
